The Distribution of Genotype and Allelic Frequency of IL28B Gene Polymorphism in Andhra Pradesh, India

• Published in: Journal of clinical and experimental hepatology 2012-06, Vol.2 (2), p.112-115
• Main Authors: Sivaprasad, Siddapuram, Rao, Padaki Nagaraja, Gupta, Rajesh, Ashwini, Kaitha, Reddy, Duvvuru Nageshwar
• Format: Article
• Language: English
• Subjects: Endocrinology & Metabolism; Gastroenterology and Hepatology; Genotype; hepatitis C virus; IL28B gene; Original; single nucleotide polymorphism
• ISSN: 0973-6883; 2213-3453
• DOI: 10.1016/S0973-6883(12)60098-X

Background The single nucleotide polymorphism (SNP) of IL28B gene on chromosome 19, encoding for the interferon (IFN)-λ-3 is strongly associated with treatment response to pegylated-IFN and ribavirin in patients infected with different genotypes of hepatitis C virus (HCV). Difference between ethnicity and treatment response rates suggesting a key role of host genetics. The IL28B polymorphism (rs12979860C/T) shows a marked differential distribution between racial groups. Aim The present study is aimed to evaluate genotype and allelic frequency of IL28B gene polymorphism (rs12979860C/T) in Andhra Pradesh, India. Methods A total of 220 healthy controls were recruited for the study. The genotyping of SNP rs12979860C/T on IL28B gene was performed by polymerase chain reaction-direct sequencing method. Result The frequency of CC genotype was found to be significantly (59.09%) higher compared to CT (34.09%) and TT (6.81%) genotypes, respectively. The frequency of major allele C is 0.762 whereas minor allele T is 0.238. Conclusion The higher distribution of genotype ‘CC’ of SNP, rs12979860C/T of IL28B gene in study subjects is suggestive of better response of HCV patients to standard anti-HCV therapy.