SNP rs8099917 in Gene IL28B Might Be Associated with Risk of Chronic Infection by HCV but Not with Response to Treatment

• Published in: BioMed research international 2014-01, Vol.2014 (2014), p.1-6
• Main Authors: da Silva Conde, Simone Regina Souza, Soares Monteiro, Julius Caesar Mendes, Silva dos Santos, Bruna Tereza, Fonseca Filgueiras, Nathália Karla, Almeida Lins, Pedro Alves de, Bonfim Freitas, Felipe, da Silva Graça, Ednelza, Demachki, Sâmia, Ferreira de Araújo, Marialva Tereza, Ishak, Ricardo, Rosário Vallinoto, Antonio Carlos
• Format: Article
• Language: English
• Subjects: Care and treatment; Case-Control Studies; Cytokines; Female; Follow-Up Studies; Gene Frequency; Genetic aspects; Genetic Association Studies; Genetic Predisposition to Disease; Genotype & phenotype; Health aspects; Hepacivirus - physiology; Hepatitis; Hepatitis C; Hepatitis C virus; Hepatitis C, Chronic - drug therapy; Hepatitis C, Chronic - genetics; Hepatitis C, Chronic - virology; Hepatology; HIV; Human immunodeficiency virus; Humans; Infections; Interferons - therapeutic use; Interleukins; Interleukins - genetics; Laboratories; Male; Middle Aged; Polymorphism, Single Nucleotide - genetics; Ribavirin - therapeutic use; Risk Factors; Single nucleotide polymorphisms; Studies; Treatment Outcome; Viral infections
• ISSN: 2314-6133; 2314-6141
• DOI: 10.1155/2014/748606

Aim. The aim of this study was to characterize the genetic profile of patients with chronic hepatitis C virus (HCV) infection relative to polymorphisms rs12979860 and rs8099917 in gene IL28B and the association of those polymorphisms with the response to treatment with pegylated interferon and ribavirin, performed at a reference center in Brazilian Amazonia. Methods. A total of 75 individuals with chronic hepatitis C and 98 healthy individuals from both genders over 18 years old were assessed. DNA samples were collected from leukocytes and subjected to real-time polymerase chain reaction to genotype polymorphisms rs12979860 and rs8099917. Results. Analysis of the allelic and genotypic frequencies of the investigated polymorphisms showed that both groups were in Hardy-Weinberg equilibrium; polymorphism rs12979860 exhibited no significant difference between the groups. For polymorphism rs8099917, allele T was significantly less frequent (P=0.0195) among the patients (63.3%) than the controls (75.5%), and the patients were 1.7 times as likely to exhibit allele G. No difference in response to treatment was associated with SNP patterns. Conclusion. The results suggest a possible association of SNP rs8099917 with higher odds of chronic HCV infection but do not indicate a putative influence of the investigated SNPs on the sustained virologic response.