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Vanadium Ions Stimulate DNA Synthesis in Swiss Mouse 3T3 and 3T6 Cells

Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 μ M are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3H]thymidine incorporation and DNA synthe...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1983-10, Vol.80 (20), p.6162-6166
Main Author: Smith, Jeffrey Bingham
Format: Article
Language:English
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Summary:Vanadyl sulfate and sodium orthovanadate in the concentration range between 5 and 50 μ M are shown to be mitogenic for quiescent cultures of Swiss mouse 3T3 and 3T6 cells. The compounds caused a striking shift in the dose-response for the effect of serum on [3H]thymidine incorporation and DNA synthesis. In the absence of serum the effect of vanadium was greatly potentiated by insulin. Vanadium ions produced no more than additive increases in [3H]thymidine incorporation when combined with epidermal growth factor, cholera toxin, or phorbol 12-myristate 13-acetate. Both vanadium compounds stimulated ouabaininhibitable86Rb+uptake, indicating that the vanadium ions increase, rather than inhibit, Na+/K+pump activity in the intact cell. Neither vanadium compound had any effect on cellular cAMP under a variety of different conditions. The mitogenic effect of the vanadium compounds was similar to that of colchicine. Taxol, which stabilizes cytoplasmic microtubules, prevented the stimulation of DNA synthesis by vanadium.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.80.20.6162