Anti-cancer effects of Grailsine-Al-glycoside isolated from Rhizoma Sparganii

An embryonic toxicity of Rhizoma sparganii was observed in mice. This study was aimed to evaluate the anticancer effects of Grailsine-Al-glycoside, the bioactive component of Rhizoma sparganii, on estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) cancer cell lines. After A549, He...

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Bibliographic Details
Published in:BMC complementary and alternative medicine 2014-03, Vol.14 (1), p.82-82
Main Authors: Zhang, Jun-Wu, Wei, Ya-Hui
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Breast cancer
Cancer therapies
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Shape - drug effects
Chinese medicine
Chromatography
Drug dosages
Drug Screening Assays, Antitumor
Drugs, Chinese Herbal - pharmacology
Estrogens
Flow cytometry
Gene expression
Mice
Morphology
Reproductive system
Rhizome - chemistry
Studies
Tumors
Typhaceae - chemistry
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Summary:An embryonic toxicity of Rhizoma sparganii was observed in mice. This study was aimed to evaluate the anticancer effects of Grailsine-Al-glycoside, the bioactive component of Rhizoma sparganii, on estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) cancer cell lines. After A549, HeLa, HepG-2 and MCF-7 cells were treated with Grailsine-Al-glycoside, cell proliferation was analyzed by MTT, cell cycle and apoptosis by flow cytometry, and morphology with an immunofluorescence microscope. Grailsine-Al-glycoside strongly suppressed cell proliferation in a dose-dependent fashion in A549, MCF-7, HepG2, and HeLa cells, though this growth inhibitory effect on HepG2 cells was not as strong and long lasting. Compared to the control, Grailsine-Al-glycoside caused a significant increase of apoptosis in A549, MCF-7 and Hela cells. A549 and MCF-7 cells were arrested at the G2/S phase whereas HepG2 cells were arrested at the G1 phase by a high concentration of Grailsine-Al-glycoside . Cell shapes were also changed by the presence of Grailsine-Al-glycoside. Grailsine-Al-glycoside from Rhizoma sparganii inhibited the proliferation of ER+ and some ER- cancer cells. Grailsine-Al-glycoside may be used as a chemotherapeutic agent against ER+ and ERRĪ±-expressing ER- cancers.
ISSN:1472-6882
1472-6882
DOI:10.1186/1472-6882-14-82