Anti-cancer effects of Grailsine-Al-glycoside isolated from Rhizoma Sparganii

An embryonic toxicity of Rhizoma sparganii was observed in mice. This study was aimed to evaluate the anticancer effects of Grailsine-Al-glycoside, the bioactive component of Rhizoma sparganii, on estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) cancer cell lines. After A549, He...

Full description

Saved in:
Bibliographic Details
Published in:BMC complementary and alternative medicine 2014-03, Vol.14 (1), p.82-82
Main Authors: Zhang, Jun-Wu, Wei, Ya-Hui
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Breast cancer
Cancer therapies
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Shape - drug effects
Chinese medicine
Chromatography
Drug dosages
Drug Screening Assays, Antitumor
Drugs, Chinese Herbal - pharmacology
Estrogens
Flow cytometry
Gene expression
Mice
Morphology
Reproductive system
Rhizome - chemistry
Studies
Tumors
Typhaceae - chemistry
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 82
container_issue 1
container_start_page 82
container_title BMC complementary and alternative medicine
container_volume 14
creator Zhang, Jun-Wu
Wei, Ya-Hui
description An embryonic toxicity of Rhizoma sparganii was observed in mice. This study was aimed to evaluate the anticancer effects of Grailsine-Al-glycoside, the bioactive component of Rhizoma sparganii, on estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) cancer cell lines. After A549, HeLa, HepG-2 and MCF-7 cells were treated with Grailsine-Al-glycoside, cell proliferation was analyzed by MTT, cell cycle and apoptosis by flow cytometry, and morphology with an immunofluorescence microscope. Grailsine-Al-glycoside strongly suppressed cell proliferation in a dose-dependent fashion in A549, MCF-7, HepG2, and HeLa cells, though this growth inhibitory effect on HepG2 cells was not as strong and long lasting. Compared to the control, Grailsine-Al-glycoside caused a significant increase of apoptosis in A549, MCF-7 and Hela cells. A549 and MCF-7 cells were arrested at the G2/S phase whereas HepG2 cells were arrested at the G1 phase by a high concentration of Grailsine-Al-glycoside . Cell shapes were also changed by the presence of Grailsine-Al-glycoside. Grailsine-Al-glycoside from Rhizoma sparganii inhibited the proliferation of ER+ and some ER- cancer cells. Grailsine-Al-glycoside may be used as a chemotherapeutic agent against ER+ and ERRα-expressing ER- cancers.
doi_str_mv 10.1186/1472-6882-14-82
format article
fullrecord <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3943449</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A539598098</galeid><sourcerecordid>A539598098</sourcerecordid><originalsourceid>FETCH-LOGICAL-b451t-a34f158466f157126790e6250bd80cfaf44c12d8f1d9b00dd95301a4df0479ff3</originalsourceid><addsrcrecordid>eNp1kttrFTEQxoMotlaffZOFQvElNdfd5EU4LVoLFcHLc8jmck5KNjludoX615ultT1HKnmYYeab34QvAeA1RqcYi_YdZh2BrRAEYgYFeQIO7ytPd_ID8KKUa4RwJzB7Dg4I40Iiig_B51WaAjQ6GTc2zntnptJk31yMOsQSkoOrCNfxxuQSrGtCyVFPzjZ-zEPzdRN-50E337Z6XOsUwkvwzOtY3Ku7eAR-fPzw_fwTvPpycXm-uoI943iCmjKPuWBtW0OHSdtJ5FrCUW8FMl57xgwmVnhsZY-QtZJThDWzHrFOek-PwPtb7nbuB2eNS9Ooo9qOYdDjjco6qP1OChu1zr8UlYwyJivg7BbQh_wfwH7H5EEtdqrFzpopQSrk7d0txvxzdmVSQyjGxaiTy3NRmCNOmGRMVOnxP9LrPI-perSoWNtRwdsH1VpHp0Lyue42C1StOJVcCiQX1ukjqnqsG4LJyflQ63sDJzsDG6fjtKnvOE8hp7IvfLNr670df78L_QNJlb-e</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1504673856</pqid></control><display><type>article</type><title>Anti-cancer effects of Grailsine-Al-glycoside isolated from Rhizoma Sparganii</title><source>Open Access: PubMed Central</source><creator>Zhang, Jun-Wu ; Wei, Ya-Hui</creator><creatorcontrib>Zhang, Jun-Wu ; Wei, Ya-Hui</creatorcontrib><description>An embryonic toxicity of Rhizoma sparganii was observed in mice. This study was aimed to evaluate the anticancer effects of Grailsine-Al-glycoside, the bioactive component of Rhizoma sparganii, on estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) cancer cell lines. After A549, HeLa, HepG-2 and MCF-7 cells were treated with Grailsine-Al-glycoside, cell proliferation was analyzed by MTT, cell cycle and apoptosis by flow cytometry, and morphology with an immunofluorescence microscope. Grailsine-Al-glycoside strongly suppressed cell proliferation in a dose-dependent fashion in A549, MCF-7, HepG2, and HeLa cells, though this growth inhibitory effect on HepG2 cells was not as strong and long lasting. Compared to the control, Grailsine-Al-glycoside caused a significant increase of apoptosis in A549, MCF-7 and Hela cells. A549 and MCF-7 cells were arrested at the G2/S phase whereas HepG2 cells were arrested at the G1 phase by a high concentration of Grailsine-Al-glycoside . Cell shapes were also changed by the presence of Grailsine-Al-glycoside. Grailsine-Al-glycoside from Rhizoma sparganii inhibited the proliferation of ER+ and some ER- cancer cells. Grailsine-Al-glycoside may be used as a chemotherapeutic agent against ER+ and ERRα-expressing ER- cancers.</description><identifier>ISSN: 1472-6882</identifier><identifier>EISSN: 1472-6882</identifier><identifier>DOI: 10.1186/1472-6882-14-82</identifier><identifier>PMID: 24589031</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Angiogenesis ; Animals ; Antineoplastic Agents - pharmacology ; Apoptosis ; Apoptosis - drug effects ; Breast cancer ; Cancer therapies ; Cell cycle ; Cell growth ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Shape - drug effects ; Chinese medicine ; Chromatography ; Drug dosages ; Drug Screening Assays, Antitumor ; Drugs, Chinese Herbal - pharmacology ; Estrogens ; Flow cytometry ; Gene expression ; Mice ; Morphology ; Reproductive system ; Rhizome - chemistry ; Studies ; Tumors ; Typhaceae - chemistry</subject><ispartof>BMC complementary and alternative medicine, 2014-03, Vol.14 (1), p.82-82</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>2014 Zhang and Wei; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.</rights><rights>Copyright © 2014 Zhang and Wei; licensee BioMed Central Ltd. 2014 Zhang and Wei; licensee BioMed Central Ltd.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943449/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3943449/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24589031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jun-Wu</creatorcontrib><creatorcontrib>Wei, Ya-Hui</creatorcontrib><title>Anti-cancer effects of Grailsine-Al-glycoside isolated from Rhizoma Sparganii</title><title>BMC complementary and alternative medicine</title><addtitle>BMC Complement Altern Med</addtitle><description>An embryonic toxicity of Rhizoma sparganii was observed in mice. This study was aimed to evaluate the anticancer effects of Grailsine-Al-glycoside, the bioactive component of Rhizoma sparganii, on estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) cancer cell lines. After A549, HeLa, HepG-2 and MCF-7 cells were treated with Grailsine-Al-glycoside, cell proliferation was analyzed by MTT, cell cycle and apoptosis by flow cytometry, and morphology with an immunofluorescence microscope. Grailsine-Al-glycoside strongly suppressed cell proliferation in a dose-dependent fashion in A549, MCF-7, HepG2, and HeLa cells, though this growth inhibitory effect on HepG2 cells was not as strong and long lasting. Compared to the control, Grailsine-Al-glycoside caused a significant increase of apoptosis in A549, MCF-7 and Hela cells. A549 and MCF-7 cells were arrested at the G2/S phase whereas HepG2 cells were arrested at the G1 phase by a high concentration of Grailsine-Al-glycoside . Cell shapes were also changed by the presence of Grailsine-Al-glycoside. Grailsine-Al-glycoside from Rhizoma sparganii inhibited the proliferation of ER+ and some ER- cancer cells. Grailsine-Al-glycoside may be used as a chemotherapeutic agent against ER+ and ERRα-expressing ER- cancers.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>Cell cycle</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Shape - drug effects</subject><subject>Chinese medicine</subject><subject>Chromatography</subject><subject>Drug dosages</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Estrogens</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Mice</subject><subject>Morphology</subject><subject>Reproductive system</subject><subject>Rhizome - chemistry</subject><subject>Studies</subject><subject>Tumors</subject><subject>Typhaceae - chemistry</subject><issn>1472-6882</issn><issn>1472-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNp1kttrFTEQxoMotlaffZOFQvElNdfd5EU4LVoLFcHLc8jmck5KNjludoX615ultT1HKnmYYeab34QvAeA1RqcYi_YdZh2BrRAEYgYFeQIO7ytPd_ID8KKUa4RwJzB7Dg4I40Iiig_B51WaAjQ6GTc2zntnptJk31yMOsQSkoOrCNfxxuQSrGtCyVFPzjZ-zEPzdRN-50E337Z6XOsUwkvwzOtY3Ku7eAR-fPzw_fwTvPpycXm-uoI943iCmjKPuWBtW0OHSdtJ5FrCUW8FMl57xgwmVnhsZY-QtZJThDWzHrFOek-PwPtb7nbuB2eNS9Ooo9qOYdDjjco6qP1OChu1zr8UlYwyJivg7BbQh_wfwH7H5EEtdqrFzpopQSrk7d0txvxzdmVSQyjGxaiTy3NRmCNOmGRMVOnxP9LrPI-perSoWNtRwdsH1VpHp0Lyue42C1StOJVcCiQX1ukjqnqsG4LJyflQ63sDJzsDG6fjtKnvOE8hp7IvfLNr670df78L_QNJlb-e</recordid><startdate>20140303</startdate><enddate>20140303</enddate><creator>Zhang, Jun-Wu</creator><creator>Wei, Ya-Hui</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140303</creationdate><title>Anti-cancer effects of Grailsine-Al-glycoside isolated from Rhizoma Sparganii</title><author>Zhang, Jun-Wu ; Wei, Ya-Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b451t-a34f158466f157126790e6250bd80cfaf44c12d8f1d9b00dd95301a4df0479ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>Cell cycle</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Shape - drug effects</topic><topic>Chinese medicine</topic><topic>Chromatography</topic><topic>Drug dosages</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Estrogens</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Mice</topic><topic>Morphology</topic><topic>Reproductive system</topic><topic>Rhizome - chemistry</topic><topic>Studies</topic><topic>Tumors</topic><topic>Typhaceae - chemistry</topic><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jun-Wu</creatorcontrib><creatorcontrib>Wei, Ya-Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest - Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>BMC complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jun-Wu</au><au>Wei, Ya-Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-cancer effects of Grailsine-Al-glycoside isolated from Rhizoma Sparganii</atitle><jtitle>BMC complementary and alternative medicine</jtitle><addtitle>BMC Complement Altern Med</addtitle><date>2014-03-03</date><risdate>2014</risdate><volume>14</volume><issue>1</issue><spage>82</spage><epage>82</epage><pages>82-82</pages><issn>1472-6882</issn><eissn>1472-6882</eissn><abstract>An embryonic toxicity of Rhizoma sparganii was observed in mice. This study was aimed to evaluate the anticancer effects of Grailsine-Al-glycoside, the bioactive component of Rhizoma sparganii, on estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) cancer cell lines. After A549, HeLa, HepG-2 and MCF-7 cells were treated with Grailsine-Al-glycoside, cell proliferation was analyzed by MTT, cell cycle and apoptosis by flow cytometry, and morphology with an immunofluorescence microscope. Grailsine-Al-glycoside strongly suppressed cell proliferation in a dose-dependent fashion in A549, MCF-7, HepG2, and HeLa cells, though this growth inhibitory effect on HepG2 cells was not as strong and long lasting. Compared to the control, Grailsine-Al-glycoside caused a significant increase of apoptosis in A549, MCF-7 and Hela cells. A549 and MCF-7 cells were arrested at the G2/S phase whereas HepG2 cells were arrested at the G1 phase by a high concentration of Grailsine-Al-glycoside . Cell shapes were also changed by the presence of Grailsine-Al-glycoside. Grailsine-Al-glycoside from Rhizoma sparganii inhibited the proliferation of ER+ and some ER- cancer cells. Grailsine-Al-glycoside may be used as a chemotherapeutic agent against ER+ and ERRα-expressing ER- cancers.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>24589031</pmid><doi>10.1186/1472-6882-14-82</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1472-6882
ispartof BMC complementary and alternative medicine, 2014-03, Vol.14 (1), p.82-82
issn 1472-6882
1472-6882
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3943449
source Open Access: PubMed Central
subjects Angiogenesis
Animals
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Breast cancer
Cancer therapies
Cell cycle
Cell growth
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Shape - drug effects
Chinese medicine
Chromatography
Drug dosages
Drug Screening Assays, Antitumor
Drugs, Chinese Herbal - pharmacology
Estrogens
Flow cytometry
Gene expression
Mice
Morphology
Reproductive system
Rhizome - chemistry
Studies
Tumors
Typhaceae - chemistry
title Anti-cancer effects of Grailsine-Al-glycoside isolated from Rhizoma Sparganii
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T08%3A50%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anti-cancer%20effects%20of%20Grailsine-Al-glycoside%20isolated%20from%20Rhizoma%20Sparganii&rft.jtitle=BMC%20complementary%20and%20alternative%20medicine&rft.au=Zhang,%20Jun-Wu&rft.date=2014-03-03&rft.volume=14&rft.issue=1&rft.spage=82&rft.epage=82&rft.pages=82-82&rft.issn=1472-6882&rft.eissn=1472-6882&rft_id=info:doi/10.1186/1472-6882-14-82&rft_dat=%3Cgale_pubme%3EA539598098%3C/gale_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b451t-a34f158466f157126790e6250bd80cfaf44c12d8f1d9b00dd95301a4df0479ff3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1504673856&rft_id=info:pmid/24589031&rft_galeid=A539598098&rfr_iscdi=true