Nuclear factor RIP140 modulates transcriptional activation by the estrogen receptor

A conserved region in the hormone‐dependent activation domain AF2 of nuclear receptors plays an important role in transcriptional activation. We have characterized a novel nuclear protein, RIP140, that specifically interacts in vitro with this domain of the estrogen receptor. This interaction was in...

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Bibliographic Details
Published in:The EMBO journal 1995-08, Vol.14 (15), p.3741-3751
Main Authors: Cavaillès, V., Dauvois, S., L'Horset, F., Lopez, G., Hoare, S., Kushner, P. J., Parker, M. G.
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing
Amino Acid Sequence
Breast Neoplasms
Cell Line
Cell Nucleus - metabolism
Cloning, Molecular
Cytoplasm - metabolism
DNA, Complementary - genetics
Estradiol - metabolism
Estradiol - pharmacology
Estrogen Antagonists - pharmacology
Humans
Molecular Sequence Data
Nuclear Proteins - chemistry
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Nuclear Receptor Interacting Protein 1
Receptors, Estrogen - genetics
Receptors, Estrogen - metabolism
Recombinant Fusion Proteins - metabolism
RNA, Messenger - biosynthesis
Sequence Analysis, DNA
Sequence Deletion
Tamoxifen - analogs & derivatives
Tamoxifen - pharmacology
Transcription Factor TFIIB
Transcription Factors - metabolism
Transcriptional Activation - physiology
Tumor Cells, Cultured
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Description
Summary:A conserved region in the hormone‐dependent activation domain AF2 of nuclear receptors plays an important role in transcriptional activation. We have characterized a novel nuclear protein, RIP140, that specifically interacts in vitro with this domain of the estrogen receptor. This interaction was increased by estrogen, but not by anti‐estrogens and the in vitro binding capacity of mutant receptors correlates with their ability to stimulate transcription. RIP140 also interacts with estrogen receptor in intact cells and modulates its transcriptional activity in the presence of estrogen, but not the anti‐estrogen 4‐hydroxytamoxifen. In view of its widespread expression in mammalian cells, RIP140 may interact with other members of the superfamily of nuclear receptors and thereby act as a potential co‐activator of hormone‐regulated gene transcription.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1995.tb00044.x