Dietary fucoxanthin increases metabolic rate and upregulated mRNA expressions of the PGC-1alpha network, mitochondrial biogenesis and fusion genes in white adipose tissues of mice

• Published in: Marine drugs 2014-02, Vol.12 (2), p.964-982
• Main Authors: Wu, Meng-Ting, Chou, Hong-Nong, Huang, Ching-jang
• Format: Article
• Language: English
• Subjects: Adipose Tissue, White - drug effects; Adipose Tissue, White - metabolism; Animals; Carbon Dioxide - metabolism; Diet; Male; Marine; Mice; Mice, Inbred C57BL; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondrial Dynamics - drug effects; Oxygen Consumption - drug effects; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; RNA, Messenger - metabolism; Transcription Factors - genetics; Up-Regulation - drug effects; Xanthophylls - administration & dosage; Xanthophylls - pharmacology
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md12020964

The mechanism for how fucoxanthin (FX) suppressed adipose accumulation is unclear. We aim to investigate the effects of FX on metabolic rate and expressions of genes related to thermogenesis, mitochondria biogenesis and homeostasis. Using a 2 × 2 factorial design, four groups of mice were respectively fed a high sucrose (50% sucrose) or a high-fat diet (23% butter + 7% soybean oil) supplemented with or without 0.2% FX. FX significantly increased oxygen consumption and carbon dioxide production and reduced white adipose tissue (WAT) mass. The mRNA expressions of peroxisome proliferator-activated receptor (PPAR) γ coactivator-1α (PGC-1α), cell death-inducing DFFA-like effecter a (CIDEA), PPARα, PPARγ, estrogen-related receptor α (ERRα), β3-adrenergic receptor (β3-AR) and deiodinase 2 (Dio2) were significantly upregulated in inguinal WAT (iWAT) and epididymal WAT (eWAT) by FX. Mitochondrial biogenic genes, nuclear respiratory factor 1 (NRF1) and NRF2, were increased in eWAT by FX. Noticeably, FX upregulated genes of mitochondrial fusion, mitofusin 1 (Mfn1), Mfn2 and optic atrophy 1 (OPA1), but not mitochondrial fission, Fission 1, in both iWAT and eWAT. In conclusion, dietary FX enhanced the metabolic rate and lowered adipose mass irrespective of the diet. These were associated with upregulated genes of the PGC-1α network and mitochondrial fusion in eWAT and iWAT.