Deoxyribozyme Cascade for Visual Detection of Bacterial RNA

In the blink of the eye: A cascade of two deoxyribozymes was designed for rapid visual detection of bacterial 16S rRNA. The detection limit is 12.5 ng by the naked eye, with the ability to differentiate between closely related pathogenic and nonpathogenic species.

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2013-11, Vol.14 (16), p.2087-2090
Main Authors: Gerasimova, Yulia V., Cornett, Evan M., Edwards, Emily, Su, Xiaoli, Rohde, Kyle H., Kolpashchikov, Dmitry M.
Format: Article
Language:English
Subjects:
3,3'-Diaminobenzidine - chemistry
Bacteria
Bacteria - genetics
Base Sequence
binary deoxyribozyme
Colorimetry
DNA, Catalytic - metabolism
Humans
mycobacterium tuberculosis
Mycobacterium tuberculosis - genetics
Oligonucleotides - chemistry
RNA recognition
RNA, Bacterial - analysis
RNA, Ribosomal, 16S - analysis
sensors
visual assays
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Description
Summary:In the blink of the eye: A cascade of two deoxyribozymes was designed for rapid visual detection of bacterial 16S rRNA. The detection limit is 12.5 ng by the naked eye, with the ability to differentiate between closely related pathogenic and nonpathogenic species.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201300471