Microfluidic assay of hemophilic blood clotting: distinct deficits in platelet and fibrin deposition at low factor levels

Summary Background Coagulation factor deficiencies create a range of bleeding phenotypes. Microfluidic devices offer controlled hemodynamics and defined procoagulant triggers for measurement of clotting under flow. Objectives We tested a flow assay of contact pathway‐triggered clotting to quantify p...

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Bibliographic Details
Published in:Journal of thrombosis and haemostasis 2014-02, Vol.12 (2), p.147-158
Main Authors: COLACE, T. V., Fogarty, P. F., Panckeri, K. A., Li, R., Diamond, S. L.
Format: Article
Language:English
Subjects:
Blood Coagulation
Blood Platelets - metabolism
Fibrin - metabolism
hemophilia
Hemophilia A - physiopathology
hemostasis
Humans
microfluidics
Microfluidics - methods
platelets
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Summary:Summary Background Coagulation factor deficiencies create a range of bleeding phenotypes. Microfluidic devices offer controlled hemodynamics and defined procoagulant triggers for measurement of clotting under flow. Objectives We tested a flow assay of contact pathway‐triggered clotting to quantify platelet and fibrin deposition distal of dysfunctional thrombin production. Microfluidic metrics were then compared with PTT or % factor activity assays. Methods Whole blood (WB) treated with low level corn trypsin inhibitor (4 μg mL−1) from nine healthy donors and 27 patients (deficient in factor [F] VIII, 19 patients; FIX, one patient; FXI, one patient; VWF, six patients) was perfused over fibrillar collagen at wall shear rate = 100 s−1. Results Using healthy WB, platelets deposited within 30 s, while fibrin appeared within 6 min. Compared with healthy controls, WB from patients displayed a 50% reduction in platelet deposition only at
ISSN:1538-7933
1538-7836
1538-7836
DOI:10.1111/jth.12457