Histone Deacetylase 3 orchestrates commensal bacteria-dependent intestinal homeostasis

• Published in: Nature (London) 2013-11, Vol.504 (7478), p.153-157
• Main Authors: Alenghat, Theresa, Osborne, Lisa C., Saenz, Steven A., Kobuley, Dmytro, Ziegler, Carly G. K., Mullican, Shannon E., Choi, Inchan, Grunberg, Stephanie, Sinha, Rohini, Wynosky-Dolfi, Meghan, Snyder, Annelise, Giacomin, Paul R., Joyce, Karen L., Hoang, Tram B., Bewtra, Meenakshi, Brodsky, Igor E., Sonnenberg, Gregory F., Bushman, Frederic D., Won, Kyoung-Jae, Lazar, Mitchell A., Artis, David
• Format: Article
• Language: English
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/nature12687

The development and severity of inflammatory bowel diseases (IBD) and other chronic inflammatory conditions can be influenced by host genetic and environmental factors, including signals derived from commensal bacteria 1 – 6 . However, the mechanisms that integrate these diverse cues remain undefined. Here we demonstrate that mice with an intestinal epithelial cell-specific deletion of the epigenome-modifying enzyme histone deacetylase 3 (HDAC3 ΔIEC mice) exhibited extensive dysregulation of IEC-intrinsic gene expression, including decreased basal expression of genes associated with antimicrobial defense. Critically, conventionally-housed HDAC3 ΔIEC mice demonstrated loss of Paneth cells, impaired IEC function and alterations in the composition of intestinal commensal bacteria. In addition, HDAC3 ΔIEC mice exhibited significantly increased susceptibility to intestinal damage and inflammation, indicating that epithelial expression of HDAC3 plays a central role in maintaining intestinal homeostasis. Rederivation of HDAC3 ΔIEC mice into germ-free conditions revealed that dysregulated IEC gene expression, Paneth cell homeostasis, and intestinal barrier function were largely restored in the absence of commensal bacteria. While the specific mechanisms through which IEC-intrinsic HDAC3 expression regulates these complex phenotypes remain to be elucidated, these data indicate that HDAC3 is a critical factor that integrates commensal bacteria-derived signals to calibrate epithelial cell responses required to establish normal host-commensal relationships and maintain intestinal homeostasis.