Agonist-specific coupling of a cloned Drosophila octopamine/tyramine receptor to multiple second messenger systems

A cloned seven transmembrane-spanning Drosophila octopamine/tyramine receptor, permanently expressed in a Chinese hamster ovary cell line, both inhibits adenylate cyclase activity and leads to the elevation of intracellular Ca2+ levels by separate G-protein-coupled pathways. Agonists of this recepto...

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Bibliographic Details
Published in:The EMBO journal 1994-03, Vol.13 (6), p.1325-1330
Main Authors: Robb, S, Cheek, T.R, Hannan, F.L, Hall, L.M, Midgley, J.M, Evans, P.D
Format: Article
Language:English
Subjects:
Adenoviridae
adenylate cyclase
Animals
binding proteins
Biological and medical sciences
calcium
Calcium - metabolism
Cell receptors
Cell structures and functions
CHO Cells
Cloning, Molecular
Cricetinae
cyclic AMP
Cyclic AMP - metabolism
Drosophila - metabolism
Drosophila melanogaster
Fundamental and applied biological sciences. Psychology
Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors
inhibition
inorganic ions
Molecular and cellular biology
octopamine
Octopamine - metabolism
Radioligand Assay
receptors
Receptors, Biogenic Amine - genetics
Receptors, Biogenic Amine - metabolism
regulation
Second Messenger Systems
tyramine
Tyramine - metabolism
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Summary:A cloned seven transmembrane-spanning Drosophila octopamine/tyramine receptor, permanently expressed in a Chinese hamster ovary cell line, both inhibits adenylate cyclase activity and leads to the elevation of intracellular Ca2+ levels by separate G-protein-coupled pathways. Agonists of this receptor (octopamine and tyramine), differing by only a single hydroxyl group in their side chain, may be capable of differentially coupling it to different second messenger systems. Thus, a single receptor may have a different pharmacological profile depending on which second messenger system is used to assay its efficacy.
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1994.tb06385.x