Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity

The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent an...

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Published in:Cellular and molecular neurobiology 2014-04, Vol.34 (3), p.323-331
Main Authors: Ghaffari, Hadi, Ghassam, Behrouz Jalali, Chandra Nayaka, S., Ramachandra Kini, K., Prakash, H. S.
Format: Article
Language:English
Subjects:
Animals
Antioxidants - pharmacology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Line, Tumor
Cell Survival - drug effects
Cell Survival - physiology
Dose-Response Relationship, Drug
Hyptis
Hyptis suaveolens
Mice
Neurobiology
Neuroprotective Agents - isolation & purification
Neuroprotective Agents - pharmacology
Neurosciences
Original Research
Oxidative Stress - drug effects
Oxidative Stress - physiology
Plant Components, Aerial
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Reactive Oxygen Species - metabolism
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Summary:The present study was carried out to investigate the antioxidant and neuroprotective effects of Hyptis suaveolens methanol extract (HSME) using various in vitro systems. The total phenol and flavonoids contents of the HSME were quantified by colorimetric methods. The HSME extract exhibited potent antioxidant activity as determined by 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazyl, and ferric reducing antioxidant power assays. The neuroprotective activity of HSME was determined on mouse N2A neuroblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lactate dehydrogenase, intracellular ROS assays, and upregulation of brain neuronal markers at genetic level. The N2A cells were pretreated with different concentrations (0.5, 1, 1.5, and 2 mg/ml) of the extract and then exposed to H 2 O 2 to induce oxidative stress and neurotoxicity. The survival of the cells treated with different concentrations of HSME and H 2 O 2 increased as compared to cells exposed only to H 2 O 2 (47.3 %) ( p  
ISSN:0272-4340
1573-6830
DOI:10.1007/s10571-013-0016-7