Sex Differences in Constitutive Autophagy

Sex bias has been described nowadays in biomedical research on animal models, although sexual dimorphism has been confirmed widely under pathological and physiological conditions. The main objective of our work was to study the sex differences in constitutive autophagy in spinal cord and skeletal mu...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-5
Main Authors: Oliván, Sara, Calvo, Ana Cristina, Manzano, Raquel, Zaragoza, Pilar, Osta, Rosario
Format: Article
Language:English
Subjects:
Animals
Autophagy
Autophagy (Cytology)
Autophagy - physiology
Biomedical research
Cancer
Demographic aspects
Female
Gender differences
Gene expression
Humans
Male
Males
Mice
Musculoskeletal system
Organ Specificity - physiology
Proteins
Rodents
Sex Characteristics
Software
Statistical analysis
Studies
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Summary:Sex bias has been described nowadays in biomedical research on animal models, although sexual dimorphism has been confirmed widely under pathological and physiological conditions. The main objective of our work was to study the sex differences in constitutive autophagy in spinal cord and skeletal muscle tissue from wild type mice. To examine the influence of sex on autophagy, mRNA and proteins were extracted from male and female mice tissues. The expressions of microtubule-associated protein 1 light chain 3 (LC3) and sequestosome 1 (p62), markers to monitor autophagy, were analyzed at 40, 60, 90, and 120 days of age. We found significant sex differences in the expression of LC3 and p62 in both tissues at these ages. The results indicated that sex and tissue specific differences exist in constitutive autophagy. These data underlined the need to include both sexes in the experimental groups to minimize any sex bias.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/652817