autonomously replicating sequence for use in a wide range of budding yeasts

The initiation of DNA replication at replication origins is essential for the duplication of genomes. In yeast, the autonomously replicating sequence (ARS) property of replication origins is necessary for the stable maintenance of episomal plasmids. However, because the sequence determinants of ARS...

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Bibliographic Details
Published in:FEMS yeast research 2014-03, Vol.14 (2), p.364-367
Main Authors: Liachko, Ivan, Dunham, Maitreya J
Format: Article
Language:English
Subjects:
Autonomously replicating sequence
DNA biosynthesis
DNA Replication
DNA, Fungal
evolution
Gene Expression
genome
Genomes
Kluyveromyces
Mutation
Pichia
Pichia pastoris
Plasmids
Plasmids - genetics
Replication initiation
Replication Origin
Replication origins
Saccharomyces
Saccharomyces cerevisiae
Saccharomycetales - classification
Saccharomycetales - genetics
Species
Yeast
yeasts
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Summary:The initiation of DNA replication at replication origins is essential for the duplication of genomes. In yeast, the autonomously replicating sequence (ARS) property of replication origins is necessary for the stable maintenance of episomal plasmids. However, because the sequence determinants of ARS function differ among yeast species, current ARS modules are limited for use to a subset of yeasts. Here, we describe a short ARS sequence that functions in at least 10 diverse species of budding yeast. These include, but are not limited to members of the Saccharomyces, Lachancea, Kluyveromyces, and Pichia (Komagataella) genera spanning over 500 million years of evolution. In addition to its wide species range, this ARS and an optimized derivative confer improved plasmid stability relative to other currently used ARS modules.
ISSN:1567-1356
1567-1364
DOI:10.1111/1567-1364.12123