Human adenovirus 36 decreases fatty acid oxidation and increases de novo lipogenesis in primary cultured human skeletal muscle cells by promoting Cidec/FSP27 expression

Background: It has been well documented that human adenovirus type 36 (Ad-36) is associated with obesity. However, the underlying molecular mechanism of Ad-36 inducing obesity remains unknown. We sought to investigate the effect of Ad-36 infection on Cidec, AMPK pathway and lipid metabolism in prima...

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Bibliographic Details
Published in:International Journal of Obesity 2010-09, Vol.34 (9), p.1355-1364
Main Authors: Wang, Z.Q, Yu, Y, Zhang, X.H, Floyd, E.Z, Cefalu, W.T
Format: Article
Language:English
Subjects:
631/443/319/2723
631/80/86
692/698/1671/1668/1973
692/699/255/2514
Adenovirus diseases
Adenoviruses
Adenoviruses, Human - genetics
Adenoviruses, Human - metabolism
Adipocytes
Biological and medical sciences
Biological oxidation (Metabolism)
Biomedical research
Body fat
cell culture
Cells, Cultured
Complications and side effects
de novo lipogenesis
Epidemiology
fatty acid composition
Fatty acids
Fatty Acids - genetics
Fatty Acids - metabolism
Genetic aspects
Glucose
Health aspects
Health Promotion and Disease Prevention
Human adenovirus D
human cell lines
Humans
Insulin resistance
Internal Medicine
Lipid Metabolism
lipid peroxidation
Lipids
lipogenesis
Lipogenesis - genetics
Lipogenesis - physiology
Medical sciences
Medicine
Medicine & Public Health
Metabolic Diseases
Metabolism
Muscle cells
Muscle, Skeletal - metabolism
Muscle, Skeletal - virology
Musculoskeletal system
Obesity
Obesity - genetics
Obesity - metabolism
Obesity - virology
original-article
Oxidation
Oxidation-Reduction
Physiological aspects
Protein expression
protein synthesis
Proteins
Proteins - genetics
Proteins - metabolism
Public Health
Risk factors
Signal Transduction
skeletal muscle
Triglycerides
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Summary:Background: It has been well documented that human adenovirus type 36 (Ad-36) is associated with obesity. However, the underlying molecular mechanism of Ad-36 inducing obesity remains unknown. We sought to investigate the effect of Ad-36 infection on Cidec, AMPK pathway and lipid metabolism in primary cultured human skeletal muscle cells. Methods: Cidec/fat-specific protein 27 (FSP27), fatty acid oxidation, AMPK signaling and the abundance of proteins involved in lipid synthesis were determined in muscle cells infected with various doses (1.9–7.6 MOI) of Ad-36 and non-lipogenic adenovirus type 2 (Ad-2) as a negative control as well as an uninfected control. Cidec/FSP27 siRNA transfection was performed in Ad-36-infected muscle cells. Results: Our data show that Ad-36 significantly reduced fatty acid oxidation in a dose-dependent manner (all P values are
ISSN:0307-0565
1476-5497
DOI:10.1038/ijo.2010.77