A Localized Wnt Signal Orients Asymmetric Stem Cell Division in Vitro

Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distributi...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2013-03, Vol.339 (6126), p.1445-1448
Main Authors: Habib, Shukry J., Chen, Bi-Chang, Tsai, Feng-Chiao, Anastassiadis, Konstantinos, Meyer, Tobias, Betzig, Eric, Nusse, Roel
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Animals
Asymmetric Cell Division
Asymmetry
beta Catenin - metabolism
Cancer
Cell Differentiation
Cell division
Cell growth
Cells
Cells, Cultured
Cellular biology
Centrosome - physiology
Centrosomes
Cytokinesis
Daughter cells
Daughters
Division
Embryonic stem cells
Embryonic Stem Cells - cytology
Embryonic Stem Cells - metabolism
Fate
Gene Expression
Genes
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Individualized Instruction
Kinases
Mice
Mitosis
Nanog Homeobox Protein
Octamer Transcription Factor-3 - genetics
Octamer Transcription Factor-3 - metabolism
Pathways
Pluripotent stem cells
Pluripotent Stem Cells - physiology
Proteins
Recombinant Proteins - metabolism
Ribonucleic acids
Signals
Single-Cell Analysis
Stem cells
Transcription Factors - genetics
Transcription Factors - metabolism
Wnt Proteins - metabolism
Wnt Signaling Pathway
Wnt-5a Protein
Wnt3A Protein - metabolism
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Summary:Developmental signals such as Wnts are often presented to cells in an oriented manner. To examine the consequences of local Wnt signaling, we immobilized Wnt proteins on beads and introduced them to embryonic stem cells in culture. At the single-cell level, the Wnt-bead induced asymmetric distribution of Wnt–β-catenin signaling components, oriented the plane of mitotic division, and directed asymmetric inheritance of centrosomes. Before cytokinesis was completed, the Wnt-proximal daughter cell expressed high levels of nuclear β-catenin and pluripotency genes, whereas the distal daughter cell acquired hallmarks of differentiation. We suggest that a spatially restricted Wnt signal induces an oriented cell division that generates distinct cell fates at predictable positions relative to the Wnt source.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1231077