Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-Gall 1

Extracts from tissue cultures of crown-gall from Parthenocissus tricuspidata (Sieb. and Zucc.) Planch. exhibited cell division activity in the soybean cytokinin assay. The chromatographic migration of one component, responsible for most of the activity, is similar to that of zeatin ribonucleoside. I...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology (Bethesda) 1971-04, Vol.47 (4), p.581-585
Main Authors: Tegley, John R., Witham, Francis H., Krasnuk, Michael
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 585
container_issue 4
container_start_page 581
container_title Plant physiology (Bethesda)
container_volume 47
creator Tegley, John R.
Witham, Francis H.
Krasnuk, Michael
description Extracts from tissue cultures of crown-gall from Parthenocissus tricuspidata (Sieb. and Zucc.) Planch. exhibited cell division activity in the soybean cytokinin assay. The chromatographic migration of one component, responsible for most of the activity, is similar to that of zeatin ribonucleoside. In addition, acid hydrolysis of the active region taken from chromatograms and of an eluate from a cation-exchange resin column resulted in the production of an active free-base-like derivative. Since the derivative and the parent compound lose activity after KMnO 4 treatment, they are believed to possess an unsaturated constituent essential for biological activity. The major active factor present in crown-gall from Parthenocissus tricuspidata is therefore distinct from the nicotinamide derivative reported to be present in Vinca rosea L. tumor cells.
format article
fullrecord <record><control><sourceid>pubmedcentral</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_396730</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>pubmedcentral_primary_oai_pubmedcentral_nih_gov_396730</sourcerecordid><originalsourceid>FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_3967303</originalsourceid><addsrcrecordid>eNqli7sOwiAYRonRaL28Ay_QBKSl7eBgiJfB0Z38KrUohQZaTd9eY1ycnb6TnPMNUERTtoyXaZIPUUTIm0meFxM0DeFGCKGMJmM0oZynGedJhA6i8q6G1l09NJU-47UF0wcdsCsxYNG37q6ttrh8Z_ioQ-gUFp1pO68-jfDuaeMdGIPpHI1KMEEtvjtDq-3mKPZx051qdTkr23owsvG6Bt9LB1r-GqsreXUPyQqeMcL-_b8A2YFWuQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-Gall 1</title><source>JSTOR Archival Journals and Primary Sources Collection</source><source>Alma/SFX Local Collection</source><creator>Tegley, John R. ; Witham, Francis H. ; Krasnuk, Michael</creator><creatorcontrib>Tegley, John R. ; Witham, Francis H. ; Krasnuk, Michael</creatorcontrib><description>Extracts from tissue cultures of crown-gall from Parthenocissus tricuspidata (Sieb. and Zucc.) Planch. exhibited cell division activity in the soybean cytokinin assay. The chromatographic migration of one component, responsible for most of the activity, is similar to that of zeatin ribonucleoside. In addition, acid hydrolysis of the active region taken from chromatograms and of an eluate from a cation-exchange resin column resulted in the production of an active free-base-like derivative. Since the derivative and the parent compound lose activity after KMnO 4 treatment, they are believed to possess an unsaturated constituent essential for biological activity. The major active factor present in crown-gall from Parthenocissus tricuspidata is therefore distinct from the nicotinamide derivative reported to be present in Vinca rosea L. tumor cells.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>PMID: 16657664</identifier><language>eng</language><ispartof>Plant physiology (Bethesda), 1971-04, Vol.47 (4), p.581-585</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885</link.rule.ids></links><search><creatorcontrib>Tegley, John R.</creatorcontrib><creatorcontrib>Witham, Francis H.</creatorcontrib><creatorcontrib>Krasnuk, Michael</creatorcontrib><title>Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-Gall 1</title><title>Plant physiology (Bethesda)</title><description>Extracts from tissue cultures of crown-gall from Parthenocissus tricuspidata (Sieb. and Zucc.) Planch. exhibited cell division activity in the soybean cytokinin assay. The chromatographic migration of one component, responsible for most of the activity, is similar to that of zeatin ribonucleoside. In addition, acid hydrolysis of the active region taken from chromatograms and of an eluate from a cation-exchange resin column resulted in the production of an active free-base-like derivative. Since the derivative and the parent compound lose activity after KMnO 4 treatment, they are believed to possess an unsaturated constituent essential for biological activity. The major active factor present in crown-gall from Parthenocissus tricuspidata is therefore distinct from the nicotinamide derivative reported to be present in Vinca rosea L. tumor cells.</description><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><recordid>eNqli7sOwiAYRonRaL28Ay_QBKSl7eBgiJfB0Z38KrUohQZaTd9eY1ycnb6TnPMNUERTtoyXaZIPUUTIm0meFxM0DeFGCKGMJmM0oZynGedJhA6i8q6G1l09NJU-47UF0wcdsCsxYNG37q6ttrh8Z_ioQ-gUFp1pO68-jfDuaeMdGIPpHI1KMEEtvjtDq-3mKPZx051qdTkr23owsvG6Bt9LB1r-GqsreXUPyQqeMcL-_b8A2YFWuQ</recordid><startdate>19710401</startdate><enddate>19710401</enddate><creator>Tegley, John R.</creator><creator>Witham, Francis H.</creator><creator>Krasnuk, Michael</creator><scope>5PM</scope></search><sort><creationdate>19710401</creationdate><title>Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-Gall 1</title><author>Tegley, John R. ; Witham, Francis H. ; Krasnuk, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_3967303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tegley, John R.</creatorcontrib><creatorcontrib>Witham, Francis H.</creatorcontrib><creatorcontrib>Krasnuk, Michael</creatorcontrib><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tegley, John R.</au><au>Witham, Francis H.</au><au>Krasnuk, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-Gall 1</atitle><jtitle>Plant physiology (Bethesda)</jtitle><date>1971-04-01</date><risdate>1971</risdate><volume>47</volume><issue>4</issue><spage>581</spage><epage>585</epage><pages>581-585</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Extracts from tissue cultures of crown-gall from Parthenocissus tricuspidata (Sieb. and Zucc.) Planch. exhibited cell division activity in the soybean cytokinin assay. The chromatographic migration of one component, responsible for most of the activity, is similar to that of zeatin ribonucleoside. In addition, acid hydrolysis of the active region taken from chromatograms and of an eluate from a cation-exchange resin column resulted in the production of an active free-base-like derivative. Since the derivative and the parent compound lose activity after KMnO 4 treatment, they are believed to possess an unsaturated constituent essential for biological activity. The major active factor present in crown-gall from Parthenocissus tricuspidata is therefore distinct from the nicotinamide derivative reported to be present in Vinca rosea L. tumor cells.</abstract><pmid>16657664</pmid></addata></record>
fulltext fulltext
identifier ISSN: 0032-0889
ispartof Plant physiology (Bethesda), 1971-04, Vol.47 (4), p.581-585
issn 0032-0889
1532-2548
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_396730
source JSTOR Archival Journals and Primary Sources Collection; Alma/SFX Local Collection
title Chromatographic Analysis of a Cytokinin from Tissue Cultures of Crown-Gall 1
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T12%3A33%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmedcentral&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chromatographic%20Analysis%20of%20a%20Cytokinin%20from%20Tissue%20Cultures%20of%20Crown-Gall%201&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Tegley,%20John%20R.&rft.date=1971-04-01&rft.volume=47&rft.issue=4&rft.spage=581&rft.epage=585&rft.pages=581-585&rft.issn=0032-0889&rft.eissn=1532-2548&rft_id=info:doi/&rft_dat=%3Cpubmedcentral%3Epubmedcentral_primary_oai_pubmedcentral_nih_gov_396730%3C/pubmedcentral%3E%3Cgrp_id%3Ecdi_FETCH-pubmedcentral_primary_oai_pubmedcentral_nih_gov_3967303%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/16657664&rfr_iscdi=true