Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

Since Teucrium chamaedrys and Teucrium montanum are the most popular plants used in the treatment of many diseases, we evaluated genotoxic potential of their methanolic extracts on cultured human peripheral blood lymphocytes (PBLs) using cytokinesis-block micronucleus (MN) assay. Cultures were treat...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2013-10, Vol.65 (5), p.863-869
Main Authors: MiloA!eviA-DjordjeviA, Olivera, StoA!iA, Ivana, StankoviA, Milan, GrujiAiA, Darko
Format: Article
Language:English
Subjects:
Biochemistry
Biomedicine
Biotechnology
Cancer therapies
Cell culture
Chemistry
Chemistry and Materials Science
Chemotherapy
Cytokinesis
Diuretics
Genotoxicity
Herbal medicine
Lymphocytes
Mitomycin C
Original Research
Peripheral blood
Teucrium
Teucrium chamaedrys
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Summary:Since Teucrium chamaedrys and Teucrium montanum are the most popular plants used in the treatment of many diseases, we evaluated genotoxic potential of their methanolic extracts on cultured human peripheral blood lymphocytes (PBLs) using cytokinesis-block micronucleus (MN) assay. Cultures were treated with four concentrations of both plants (125, 250, 500 and 1,000 μg/ml), both separately and in combination with mitomycin C (MMC). The results revealed that extract of T. chamaedrys administered at the tested concentrations did not significantly affect the mean MN frequency in comparison to untreated cells. Methanolic extract of T. montanum increased the mean MN frequency in PBL at the tested concentrations, but significantly only at the concentration of 1,000 μg/ml. In all tested concentrations, the extract of T. chamaedrys significantly reduced the MMC-induced MN frequency, in a dose dependent manner (r = − 0.687, p  
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-012-9527-1