Adenosine diphosphoglucose-starch glucosyltransferases from developing kernels of waxy maize

Two adenosine diphosphoglucose: α-1,4-glucan α-4-glucosyl-transferases were extracted from kernels of waxy maize harvested 22 days after pollination and separated by gradient elution from a diethylaminoethyl-cellulose column. Both fractions could utilize amylopectin, amylose, glycogen, maltotriose a...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1971-12, Vol.48 (6), p.765-769
Main Authors: Ozbun, J.L, Hawker, J.S, Preiss, J
Format: Article
Language:English
Subjects:
Anions
Citrates
Corn
Enzymes
field crops
Glucans
Glycogen
Iodine
plant biochemistry
plant physiology
Sodium
Spinach
Starches
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Summary:Two adenosine diphosphoglucose: α-1,4-glucan α-4-glucosyl-transferases were extracted from kernels of waxy maize harvested 22 days after pollination and separated by gradient elution from a diethylaminoethyl-cellulose column. Both fractions could utilize amylopectin, amylose, glycogen, maltotriose and maltose as primers. The rate of glucose transfer from adenosine diphosphoglucose to rabbit liver glycogen of fraction II was 78% of the rate of glucose transfer to amylopectin, but with fraction I the rate of transfer of glucose to rabbit liver glycogen was 380% of that observed to amylopectin. Glucan synthesis in the absence of added primer was found in fraction I in the presence of 0.5 M sodium citrate and bovine serum albumin. The unprimed product was a methanol-precipitable glucan with principally α-1,4 linkages and some α-1,6 linkages, and its iodine spectrum was similar to that of amylopectin.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.48.6.765