Overexpression of Human Catalase Inhibits Proliferation and Promotes Apoptosis in Vascular Smooth Muscle Cells

The role of reactive oxygen species, such as superoxide anions (O2) and hydrogen peroxide (H2O2), in modulating vascular smooth muscle cell proliferation and viability is controversial. To investigate the role of endogenously produced H2O2, rat aortic smooth muscle cells were infected with adenovira...

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Bibliographic Details
Published in:Circulation research 1999-09, Vol.85 (6), p.524-533
Main Authors: Brown, Michael R, Miller, Francis J, Li, Wei-Gen, Ellingson, Andy N, Mozena, Jonathan D, Chatterjee, Papri, Engelhardt, John F, Zwacka, Ralf M, Oberley, Larry W, Fang, Xiang, Spector, Arthur A, Weintraub, Neal L
Format: Article
Language:English
Subjects:
Animals
Apoptosis - physiology
Biological and medical sciences
Blood vessels and receptors
Catalase - genetics
Catalase - metabolism
Cell Division - physiology
Cells, Cultured
Cyclooxygenase 2
Fundamental and applied biological sciences. Psychology
Gene Transfer Techniques
Humans
Intracellular Membranes - metabolism
Isoenzymes - metabolism
Membrane Proteins
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - enzymology
Muscle, Smooth, Vascular - physiology
Prostaglandin-Endoperoxide Synthases - metabolism
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species - metabolism
Vertebrates: cardiovascular system
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Summary:The role of reactive oxygen species, such as superoxide anions (O2) and hydrogen peroxide (H2O2), in modulating vascular smooth muscle cell proliferation and viability is controversial. To investigate the role of endogenously produced H2O2, rat aortic smooth muscle cells were infected with adenoviral vectors containing cDNA for human catalase (AdCat) or a control gene, β-galactosidase (AdLacZ). Infection with AdCat resulted in dose-dependent increases in intracellular catalase protein, which was predominantly localized to peroxisomes. After infection with 100 multiplicity of infection (MOI) of AdCat, cellular catalase activity was increased by 50- to 100-fold, and intracellular H2O2 concentration was reduced, as compared with control. Infection with AdCat reduced [H]thymidine uptake, an index of DNA synthesis, in cells maintained in medium supplemented with 2% serum (0.37±0.09 disintegrations per minute per cell [AdLacZ] versus 0.22±0.08 disintegrations per minute per cell [AdCat], P
ISSN:0009-7330
1524-4571
DOI:10.1161/01.res.85.6.524