Bioengineered nanoparticles for siRNA delivery

• Published in: Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology 2013-09, Vol.5 (5), p.449-468
• Main Authors: Kozielski, Kristen L., Tzeng, Stephany Y., Green, Jordan J.
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials - chemistry; Bioengineering; Bioengineering - methods; Biomaterials; Biomedical materials; Calcium Phosphates - chemistry; Conflicts of interest; Cues; Deoxyribonucleic acid; DNA; Drug Administration Routes; Drug Delivery Systems; Drug discovery; Endosomes - metabolism; Gene expression; Gold - chemistry; Humans; Lipids - chemistry; Liposomes - chemistry; Medical treatment; Nanomedicine - methods; Nanoparticles; Nanoparticles - chemistry; Nanostructure; Nanotechnology; Polymers - chemistry; Quantum Dots; Ribonucleic acid; Ribonucleic acids; RNA; RNA - chemistry; RNA Interference; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; RNA-mediated interference; Silicon Dioxide - chemistry; siRNA; Surgical implants; Wire
• ISSN: 1939-5116; 1939-0041
• DOI: 10.1002/wnan.1233

Short interfering RNA (siRNA) has been an important laboratory tool in the last two decades and has allowed researchers to better understand the functions of nonprotein‐coding genes through RNA interference (RNAi). Although RNAi holds great promise for this purpose as well as for treatment of many diseases, efforts at using siRNA have been hampered by the difficulty of safely and effectively introducing it into cells of interest, both in vitro and in vivo. To overcome this challenge, many biomaterials and nanoparticles (NPs) have been developed and optimized for siRNA delivery, often taking cues from the DNA delivery field, although different barriers exist for these two types of molecules. In this review, we discuss general properties of biomaterials and nanoparticles that are necessary for effective nucleic acid delivery. We also discuss specific examples of bioengineered materials, including lipid‐based NPs, polymeric NPs, inorganic NPs, and RNA‐based NPs, which clearly illustrate the problems and successes in siRNA delivery. WIREs Nanomed Nanobiotechnol 2013. doi: 10.1002/wnan.1233 This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease