RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax

Continuous cell lines could provide an important tool for studying epidemiology, toxicology, cellular physiology and the host–pathogen interactions. Random amplified polymorphic deoxyribonucleic acid analysis by PCR (RAPD-PCR) was used for the molecular characterization of Dicentrarchus labrax embry...

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Bibliographic Details
Published in:Cytotechnology (Dordrecht) 2014-05, Vol.66 (3), p.383-393
Main Authors: Rocco, L., Valentino, I. V., Scapigliati, G., Stingo, V.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedicine
Biotechnology
Cell lines
Cells
Chelating agents
Chemistry
Chemistry and Materials Science
Dicentrarchus labrax
DNA fingerprints
Embryos
Epidemiology
Genomes
Genotoxicity
Host-pathogen interactions
Laboratories
Marine
Mutation
Original Research
Phenols
Pollutants
Polymerase chain reaction
Random amplified polymorphic DNA
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Summary:Continuous cell lines could provide an important tool for studying epidemiology, toxicology, cellular physiology and the host–pathogen interactions. Random amplified polymorphic deoxyribonucleic acid analysis by PCR (RAPD-PCR) was used for the molecular characterization of Dicentrarchus labrax embryonic cells (DLEC) as a possible tool to detect DNA alterations in environmental genotoxic studies. We studied the DNA pattern of the DLEC fish cell line, a fibroblast-like cell line derived from European sea bass. From a total of 15 primers only six showed good discriminatory power for the amplification process on DNA samples collected from cells by three different methods (organic extraction, salting-out method and chelating agent extraction). The results obtained show that the cell line chosen for this study could be used as a possible tool for the detection of potential genotoxicity of numerous chemical compounds.
ISSN:0920-9069
1573-0778
DOI:10.1007/s10616-013-9586-y