Role of miR-191/425 cluster in tumorigenesis and diagnosis of gastric cancer

Gastric cancer (GC) is among the most frequent types of cancer worldwide. Therefore, understanding the biology of GC tumorigenesis is important for appropriate diagnosis and patient surveillance. The miR-191/425 cluster has been reported to be overexpressed in various human cancers, but the tumorige...

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Bibliographic Details
Published in:International journal of molecular sciences 2014-03, Vol.15 (3), p.4031-4048
Main Authors: Peng, Wei-Zhao, Ma, Ren, Wang, Fang, Yu, Jia, Liu, Zhi-Bin
Format: Article
Language:English
Subjects:
Adult
Aged
Biomarkers
Biomarkers, Tumor - blood
Biomarkers, Tumor - genetics
Carcinogenesis - genetics
Cell Cycle - genetics
Cell Line, Tumor
Cell Movement - genetics
Cell Proliferation
Cell Transformation, Neoplastic - genetics
Female
Gastric cancer
Gene Expression Regulation, Neoplastic
Humans
Male
Medical diagnosis
MicroRNAs - blood
MicroRNAs - genetics
Middle Aged
Multigene Family
Reverse Transcriptase Polymerase Chain Reaction
Stomach - metabolism
Stomach - pathology
Stomach Neoplasms - blood
Stomach Neoplasms - diagnosis
Stomach Neoplasms - genetics
Tumorigenesis
Tumors
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Summary:Gastric cancer (GC) is among the most frequent types of cancer worldwide. Therefore, understanding the biology of GC tumorigenesis is important for appropriate diagnosis and patient surveillance. The miR-191/425 cluster has been reported to be overexpressed in various human cancers, but the tumorigenic role and clinical significance of miR-191/425 overexpression in gastric carcinogenesis is currently undefined. In this study, the expression of miR-191 and miR-425 in GC tissue and serum was assessed, and the relationship between miRNA expression and clinicopathological data was analyzed. We found that miR-191 and miR-425 were both significantly increased in human GC tissues relative to adjacent normal controls. In addition, miR-191 levels correlated with GC tumor stage and metastatic state. Furthermore, the level of serum miR-191 was significantly higher in the GC group than in the control group when using serum miR-16 as an endogenous control. Finally, inhibition of miR-191 or miR-425 in the GC cell lines HGC-27 not only reduced cell proliferation and cell cycle progression but also impaired cell migration and invasion. Taken together, our results revealed the oncogenic roles of miR-191 and miR-425 in gastric carcinogenesis, and indicated the potential use of serum miR-191 as a novel and stable biomarker for GC diagnosis.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms15034031