Identification and functional characterization of leukotriene B4 20-hydroxylase of human polymorphonuclear leukocytes

• Published in: Proceedings of the National Academy of Sciences - PNAS 1985-04, Vol.82 (8), p.2292-2295
• Main Authors: SOBERMAN, R. J, HARPER, T. W, MURPHY, R. C, AUSTEN, K. F
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Cytochrome P-450 Enzyme System - blood; Cytochrome P450 Family 4; Cytosol - enzymology; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Humans; Kinetics; Leukotriene B4; Mixed Function Oxygenases - blood; Neutrophils - enzymology; Oxidoreductases
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.82.8.2292

A single reaction product was formed during the incubation of 1.5 microM (5S,12R)-dihydroxy-6,14-cis-8,10-trans-[3H]icosatetraenoic acid (leukotriene B4, LTB4) for 30 min at 37 degrees C in 10 mM potassium phosphate buffer (pH 7.5) with 100 microM NADPH and the 150,000 X g supernatant of sonicated human polymorphonuclear leukocytes (PMN). The reaction product exhibited the same mobility on reversed-phase HPLC (RP-HPLC) and TLC as standard 20-hydroxy-LTB4 (20-OH-LTB4). When the omega-oxidation product of [3H]LTB4 was eluted from a Sep-Pak, resolved by RP-HPLC, and analyzed by GC/MS, its structure was determined to be solely 20-OH-LTB4. The Km of the 20-hydroxylase for [3H]LTB4 at its optimal pH of 7.5 was 0.22 +/- 0.08 microM (mean +/- SD, n = 4) and the Vmax was 48 +/- 11 pmol/min X mg of protein (mean +/- SD, n = 4). When the concentration of [3H]LTB4 was fixed at 1.5 microM, the Km for NADPH was 1.01 +/- 0.59 microM (mean +/- SD, n = 3). The location in the 150,000 X g supernatant of the LTB4 20-hydroxylase distinguishes it from the cytochrome P-450 system of liver, lung, and kidney microsomes and from the NADPH oxidase-cytochrome b-245 system of the human PMN. The LTB4 20-hydroxylase is either a unique cytochrome P-450 or other monooxygenase.