A pilot study investigating early postoperative changes of plasma polyunsaturated fatty acids after laparoscopic sleeve gastrectomy

• Published in: Lipids in health and disease 2014-04, Vol.13 (1), p.62-62, Article 62
• Main Authors: Aslan, Mutay, Aslan, Ibrahim, Özcan, Filiz, Eryılmaz, Ramazan, Ensari, Cemal Ozben, Bilecik, Tuna
• Format: Article
• Language: English
• Subjects: 8,11,14-Eicosatrienoic Acid - blood; Analysis; Arachidonic Acid - blood; Body mass index; Cardiovascular disease; Clothing and dress; Dinoprostone - blood; Docosahexaenoic Acids - blood; Eicosapentaenoic Acid - blood; Fatty acids; Fatty Acids, Unsaturated - blood; Gastrectomy; Health aspects; Humans; Insulin; Insulin - blood; Insulin resistance; Investigations; Laparoscopy; Measurement; Medical research; Medicine, Experimental; Obesity; Obesity - blood; Pilot Projects; Plasma; Prostaglandins E; Spectrometry, Mass, Electrospray Ionization; Type 2 diabetes; Weight control
• ISSN: 1476-511X; 1476-511X
• DOI: 10.1186/1476-511X-13-62

This study aimed to determine early postoperative changes of plasma polyunsaturated fatty acids (PUFAs) following laparoscopic sleeve gastrectomy (LSG). Ten obese patients (mean BMI: 51.10 ± 11.59 kg/m²) underwent LSG and eleven normal weight control patients (mean BMI: 24.37 ± 2.33 kg/m²) underwent laparoscopic abdominal surgery. Fasting blood samples were collected prior to surgery, at day 1 after surgery and after postoperation oral feeding. Plasma levels of arachidonic acid (AA, C20:4n6), dihomo-gamma-linolenic acid (DGLA, C20:3n6), eicosapentaenoic acid (EPA, C20:5n3) and docosahexaenoic acid (DHA, C22:6n3) were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Prostaglandin E₂ (PGE2) was measured in serum samples by enzyme immunoassay. A significant decrease was observed in insulin and HOMA IR levels in sleeve gastrectomy patients after postoperation oral feeding compared to preoperation. Plasma AA levels and AA/EPA ratio were significantly increased in sleeve gastrectomy patients after postoperation oral feeding compared to postoperation day 1. Serum PGE2 levels and AA/DHA ratio was significantly higher in sleeve gastrectomy patients at preoperation, postoperation day 1 and after postoperation oral feeding when compared to control group patients. Increased peripheral insulin sensitivity associated with LSG may play a role in the significant increase of plasma AA levels in sleeve gastrectomy patients following postoperation oral feeding. The significant increase in PGE2 levels and AA/DHA ratio in sleeve gastrectomy group patients also confirms the presence of a proinflammatory state in obesity.