Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells

Osteoblast differentiation plays an essential role in bone integrity. Isoflavones and some flavonoids are reported to have osteogenic activity and potentially possess the ability to treat osteoporosis. However, limited information concerning the osteogenic characteristics of hydroxyflavones is avail...

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Published in:Evidence-based complementary and alternative medicine 2014-01, Vol.2014 (2014), p.1-11
Main Authors: Lin, Yu-Hsaing, Yeh, Shauh-Der, Wu, Yu-Wei, Lai, Chien-Hung, Tsai, Yu-Hui
Format: Article
Language:English
Subjects:
AKT protein
c-Jun protein
Calcein
Cell cycle
Cytotoxicity
Extracellular signal-regulated kinase
Flavonoids
Fractures
Isoflavones
JNK protein
Kinases
Metabolism
Metabolites
Mineralization
Osteoblastogenesis
Osteoblasts
Osteoporosis
Pharmacology
Serine
Signal transduction
Threonine
Transcription factors
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cited_by cdi_FETCH-LOGICAL-c500t-88540b66c191519e6d8c9c79449b6f1bf80f7a6b31b562145e39549a018607443
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container_issue 2014
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container_title Evidence-based complementary and alternative medicine
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creator Lin, Yu-Hsaing
Yeh, Shauh-Der
Wu, Yu-Wei
Lai, Chien-Hung
Tsai, Yu-Hui
description Osteoblast differentiation plays an essential role in bone integrity. Isoflavones and some flavonoids are reported to have osteogenic activity and potentially possess the ability to treat osteoporosis. However, limited information concerning the osteogenic characteristics of hydroxyflavones is available. This study investigates the effects of various hydroxyflavones on osteoblast differentiation in MC3T3-E1 cells. The results showed that 6-hydroxyflavone (6-OH-F) and 7-hydroxyflavone (7-OH-F) stimulated ALP activity. However, baicalein and luteolin inhibited ALP activity and flavone showed no effect. Up to 50 μM of each compound was used for cytotoxic effects study; flavone, 6-OH-F, and 7-OH-F had no cytotoxicity on MC3T3-E1 cells. Moreover, 6-OH-F activated AKT and serine/threonine kinases (also known as protein kinase B or PKB), extracellular signal-regulated kinases (ERK 1/2), and the c-Jun N-terminal kinase (JNK) signaling pathways. On the other hand, 7-OH-F promoted osteoblast differentiation mainly by activating ERK 1/ 2 signaling pathways. Finally, after 5 weeks of 6-OH-F induction, MC3T3-E1 cells showed a significant increase in the calcein staining intensity relative to merely visible mineralization observed in cells cultured in the osteogenic medium only. These results suggested that 6-OH-F could activate AKT, ERK 1/2, and JNK signaling pathways to effectively promote osteoblastic differentiation.
doi_str_mv 10.1155/2014/924560
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Isoflavones and some flavonoids are reported to have osteogenic activity and potentially possess the ability to treat osteoporosis. However, limited information concerning the osteogenic characteristics of hydroxyflavones is available. This study investigates the effects of various hydroxyflavones on osteoblast differentiation in MC3T3-E1 cells. The results showed that 6-hydroxyflavone (6-OH-F) and 7-hydroxyflavone (7-OH-F) stimulated ALP activity. However, baicalein and luteolin inhibited ALP activity and flavone showed no effect. Up to 50 μM of each compound was used for cytotoxic effects study; flavone, 6-OH-F, and 7-OH-F had no cytotoxicity on MC3T3-E1 cells. Moreover, 6-OH-F activated AKT and serine/threonine kinases (also known as protein kinase B or PKB), extracellular signal-regulated kinases (ERK 1/2), and the c-Jun N-terminal kinase (JNK) signaling pathways. On the other hand, 7-OH-F promoted osteoblast differentiation mainly by activating ERK 1/ 2 signaling pathways. 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Isoflavones and some flavonoids are reported to have osteogenic activity and potentially possess the ability to treat osteoporosis. However, limited information concerning the osteogenic characteristics of hydroxyflavones is available. This study investigates the effects of various hydroxyflavones on osteoblast differentiation in MC3T3-E1 cells. The results showed that 6-hydroxyflavone (6-OH-F) and 7-hydroxyflavone (7-OH-F) stimulated ALP activity. However, baicalein and luteolin inhibited ALP activity and flavone showed no effect. Up to 50 μM of each compound was used for cytotoxic effects study; flavone, 6-OH-F, and 7-OH-F had no cytotoxicity on MC3T3-E1 cells. Moreover, 6-OH-F activated AKT and serine/threonine kinases (also known as protein kinase B or PKB), extracellular signal-regulated kinases (ERK 1/2), and the c-Jun N-terminal kinase (JNK) signaling pathways. On the other hand, 7-OH-F promoted osteoblast differentiation mainly by activating ERK 1/ 2 signaling pathways. 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subjects AKT protein
c-Jun protein
Calcein
Cell cycle
Cytotoxicity
Extracellular signal-regulated kinase
Flavonoids
Fractures
Isoflavones
JNK protein
Kinases
Metabolism
Metabolites
Mineralization
Osteoblastogenesis
Osteoblasts
Osteoporosis
Pharmacology
Serine
Signal transduction
Threonine
Transcription factors
title Effects of 6-Hydroxyflavone on Osteoblast Differentiation in MC3T3-E1 Cells
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