Gene expression related to oxidative stress in the heart of mice after intestinal ischemia

Intestinal ischemia-reperfusion is a frequent clinical event associated to injury in distant organs, especially the heart. To investigate the gene expression of oxidative stress and antioxidant defense in the heart of inbred mice subjected to intestinal ischemia and reperfusion (IR). Twelve mice (C5...

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Bibliographic Details
Published in:Arquivos brasileiros de cardiologia 2014-02, Vol.102 (2), p.165-173
Main Authors: Somaio Neto, Frederico, Ikejiri, Adauto Tsutomu, Bertoletto, Paulo Roberto, Chaves, José Carlos Bertoletto, Teruya, Roberto, Fagundes, Djalma José, Taha, Murched Omar
Format: Article
Language:English
Subjects:
Animals
Antioxidants - metabolism
Disease Models, Animal
Gene Expression - genetics
Intestine, Small - blood supply
Intestine, Small - metabolism
Ischemia - genetics
Ischemia - metabolism
Male
Mice, Inbred C57BL
Myocardium - metabolism
Original
Oxidative Stress - genetics
Random Allocation
Reperfusion Injury - genetics
Reperfusion Injury - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Up-Regulation - genetics
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Summary:Intestinal ischemia-reperfusion is a frequent clinical event associated to injury in distant organs, especially the heart. To investigate the gene expression of oxidative stress and antioxidant defense in the heart of inbred mice subjected to intestinal ischemia and reperfusion (IR). Twelve mice (C57BL/6) were assigned to: IR Group (GIR) with 60 minutes of superior mesenteric artery occlusion followed by 60 minutes of reperfusion; Control Group (CG) which underwent anesthesia and laparotomy without IR procedure and was observed for 120 minutes. Intestine and heart samples were processed using the RT-qPCR/Reverse transcriptase-quantitative Polymerase Chain Reaction method for the gene expression of 84 genes related to oxidative stress and oxidative defense (Student's "t" test, p
ISSN:0066-782X
1678-4170
DOI:10.5935/abc.20130240