Engineered Tumor Cell Apoptosis Monitoring Method Based on Dynamic Laser Tweezers

Monitoring the cells’ apoptosis progression could provide a valuable insight into the temporal events that initiate cell death as well as the potential for rescue of apoptotic cells. In this paper, we engineered a novel and robust method for monitoring apoptosis of tumor cells based on dynamic laser...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-6
Main Authors: Min, Changjun, Zhang, Yuquan, Yuan, Xiaocong, Urbach, H. Paul, Zhu, Siwei, Wu, Xiao-jing
Format: Article
Language:English
Subjects:
Antineoplastic Agents - administration & dosage
Apoptosis
Apoptosis - drug effects
Cancer
Care and treatment
Cell Line, Tumor
Cisplatin - administration & dosage
Equipment Design
Equipment Failure Analysis
Flow Cytometry - instrumentation
Flow Cytometry - methods
Genetic aspects
Health aspects
HeLa Cells
Humans
Laser therapy
Lasers
Methods
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Micromanipulation - instrumentation
Micromanipulation - methods
Neoplasms, Experimental - drug therapy
Neoplasms, Experimental - pathology
Neoplasms, Experimental - physiopathology
Optical Tweezers
Science
Tumors
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Summary:Monitoring the cells’ apoptosis progression could provide a valuable insight into the temporal events that initiate cell death as well as the potential for rescue of apoptotic cells. In this paper, we engineered a novel and robust method for monitoring apoptosis of tumor cells based on dynamic laser tweezers, using A549 and HeLa cell line as typical samples. The entire experiment can be completed in a few hours with small amount of fluid sample, presenting great advantages of celerity, microscaled measurement, and label-free explorations without perturbing experimental conditions in combination with other probes. Validity and stability of this method are verified experimentally in terms of physical parameters of the system. The proposed technique has great potential in improving cancer treatment by monitoring the objective efficacy of tumor cell killing.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/279408