Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid

[Display omitted] •SABRE polarization of two drugs.•Up to 8% polarization of proton is achieved in less than 1min.•SABRE polarization dependence on polarization field, temperature and solvent is determined. Hyperpolarization produces nuclear spin polarization that is several orders of magnitude larg...

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Published in:Journal of magnetic resonance (1997) 2013-12, Vol.237 (100), p.73-78
Main Authors: Zeng, Haifeng, Xu, Jiadi, Gillen, Joseph, McMahon, Michael T., Artemov, Dmitri, Tyburn, Jean-Max, Lohman, Joost A.B., Mewis, Ryan E., Atkinson, Kevin D., Green, Gary G.R., Duckett, Simon B., van Zijl, Peter C.M.
Format: Article
Language:English
Subjects:
Antitubercular Agents - chemistry
Electromagnetic Fields
Hydrogen - chemistry
Hyperpolarization
Isoniazid
Isoniazid - chemistry
Magnetic Resonance Spectroscopy - methods
Mycobacterium
Mycobacterium Tuberculosis
Parahydrogen
Pyrazinamide
Pyrazinamide - chemistry
SABRE
Solvents
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Summary:[Display omitted] •SABRE polarization of two drugs.•Up to 8% polarization of proton is achieved in less than 1min.•SABRE polarization dependence on polarization field, temperature and solvent is determined. Hyperpolarization produces nuclear spin polarization that is several orders of magnitude larger than that achieved at thermal equilibrium thus providing extraordinary contrast and sensitivity. As a parahydrogen induced polarization (PHIP) technique that does not require chemical modification of the substrate to polarize, Signal Amplification by Reversible Exchange (SABRE) has attracted a lot of attention. Using a prototype parahydrogen polarizer, we polarize two drugs used in the treatment of tuberculosis, namely pyrazinamide and isoniazid. We examine this approach in four solvents, methanol-d4, methanol, ethanol and DMSO and optimize the polarization transfer magnetic field strength, the temperature as well as intensity and duration of hydrogen bubbling to achieve the best overall signal enhancement and hence hyperpolarization level.
ISSN:1090-7807
1096-0856
DOI:10.1016/j.jmr.2013.09.012