Gellan sulfate inhibits Plasmodium falciparum growth and invasion of red blood cells in vitro

Here, we assessed the sulfated derivative of the microbial polysaccharide gellan gum and derivatives of λ and κ-carrageenans for their ability to inhibit Plasmodium falciparum 3D7 and Dd2 growth and invasion of red blood cells in vitro . Growth inhibition was assessed by means of flow cytometry afte...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2014-04, Vol.4 (1), p.4723
Main Authors: Recuenco, Frances Cagayat, Kobayashi, Kyousuke, Ishiwa, Akiko, Enomoto-Rogers, Yukiko, Fundador, Noreen Grace V., Sugi, Tatsuki, Takemae, Hitoshi, Iwanaga, Tatsuya, Murakoshi, Fumi, Gong, Haiyan, Inomata, Atsuko, Horimoto, Taisuke, Iwata, Tadahisa, Kato, Kentaro
Format: Article
Language:English
Subjects:
13/31
14/63
140/131
631/326/417/1716
692/308/153
692/699/255/1629
Animals
Anticoagulants
Carrageenans
Cytotoxicity
Erythrocytes
Erythrocytes - parasitology
Flow Cytometry
Gellan gum
Humanities and Social Sciences
Humans
In Vitro Techniques
Malaria, Falciparum - blood
Malaria, Falciparum - drug therapy
Malaria, Falciparum - parasitology
multidisciplinary
Parasites
Plasmodium falciparum
Plasmodium falciparum - drug effects
Plasmodium falciparum - radiation effects
Polysaccharides
Polysaccharides - blood
Polysaccharides - pharmacology
Saccharides
Science
Sulfates
Sulfuric Acid Esters - blood
Sulfuric Acid Esters - pharmacology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Here, we assessed the sulfated derivative of the microbial polysaccharide gellan gum and derivatives of λ and κ-carrageenans for their ability to inhibit Plasmodium falciparum 3D7 and Dd2 growth and invasion of red blood cells in vitro . Growth inhibition was assessed by means of flow cytometry after a 96-h exposure to the inhibitors and invasion inhibition was assessed by counting ring parasites after a 20-h exposure to them. Gellan sulfate strongly inhibited invasion and modestly inhibited growth for both P. falciparum 3D7 and Dd2; both inhibitory effects exceeded those achieved with native gellan gum. The hydrolyzed λ-carrageenan and oversulfated κ-carrageenan were less inhibitory than their native forms. In vitro cytotoxicity and anticoagulation assays performed to determine the suitability of the modified polysaccharides for in vivo studies showed that our synthesized gellan sulfate had low cytotoxicity and anticoagulant activity.
ISSN:2045-2322
DOI:10.1038/srep04723