A murine model of subarachnoid hemorrhage

In this video publication a standardized mouse model of subarachnoid hemorrhage (SAH) is presented. Bleeding is induced by endovascular Circle of Willis perforation (CWp) and proven by intracranial pressure (ICP) monitoring. Thereby a homogenous blood distribution in subarachnoid spaces surrounding...

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Bibliographic Details
Published in:Journal of visualized experiments 2013-11 (81), p.e50845-e50845
Main Authors: Schüller, Kathrin, Bühler, Dominik, Plesnila, Nikolaus
Format: Article
Language:English
Subjects:
Animals
Circle of Willis - surgery
Disease Models, Animal
Intracranial Pressure
Male
Medicine
Mice
Mice, Inbred C57BL
Subarachnoid Hemorrhage
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Summary:In this video publication a standardized mouse model of subarachnoid hemorrhage (SAH) is presented. Bleeding is induced by endovascular Circle of Willis perforation (CWp) and proven by intracranial pressure (ICP) monitoring. Thereby a homogenous blood distribution in subarachnoid spaces surrounding the arterial circulation and cerebellar fissures is achieved. Animal physiology is maintained by intubation, mechanical ventilation, and continuous on-line monitoring of various physiological and cardiovascular parameters: body temperature, systemic blood pressure, heart rate, and hemoglobin saturation. Thereby the cerebral perfusion pressure can be tightly monitored resulting in a less variable volume of extravasated blood. This allows a better standardization of endovascular filament perforation in mice and makes the whole model highly reproducible. Thus it is readily available for pharmacological and pathophysiological studies in wild type and genetically altered mice.
ISSN:1940-087X
1940-087X
DOI:10.3791/50845