Simultaneous detection and discrimination of virulent and benign Dichelobacter nodosus in sheep of flocks affected by foot rot and in clinically healthy flocks by competitive real-time PCR

Ovine foot rot caused by Dichelobacter nodosus is affecting sheep worldwide. The current diagnostic methods are difficult and cumbersome. Here, we present a competitive real-time PCR based on allelic discrimination of the protease genes aprV2 and aprB2. This method allows direct detection and differ...

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Bibliographic Details
Published in:Journal of clinical microbiology 2014-04, Vol.52 (4), p.1228-1231
Main Authors: Stäuble, Anna, Steiner, Adrian, Frey, Joachim, Kuhnert, Peter
Format: Article
Language:English
Subjects:
Animals
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Bacterial Typing Techniques - methods
Clinical Veterinary Microbiology
Dichelobacter nodosus
Dichelobacter nodosus - isolation & purification
Foot Rot - diagnosis
Foot Rot - microbiology
Real-Time Polymerase Chain Reaction - methods
Serine Endopeptidases - analysis
Serine Endopeptidases - genetics
Sheep
Sheep Diseases - diagnosis
Sheep Diseases - microbiology
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Summary:Ovine foot rot caused by Dichelobacter nodosus is affecting sheep worldwide. The current diagnostic methods are difficult and cumbersome. Here, we present a competitive real-time PCR based on allelic discrimination of the protease genes aprV2 and aprB2. This method allows direct detection and differentiation of virulent and benign D. nodosus from interdigital skin swabs in a single test. Clinically affected sheep harbored high loads of only virulent strains, whereas healthy sheep had lower loads of predominantly benign strains.
ISSN:0095-1137
1098-660X
DOI:10.1128/JCM.03485-13