Non-mulberry silk fibroin influence osteogenesis and osteoblast-macrophage cross talk on titanium based surface

The titanium and its alloys are used as orthopedic dental implants due to their mechanical and bio-inert properties. The bare metal implants are not the ultimate answer for better osteogenesis and implant integration. Physical and chemical modifications are carried out to achieve the goal of improve...

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Bibliographic Details
Published in:Scientific reports 2014-04, Vol.4 (1), p.4745-4745, Article 4745
Main Authors: Naskar, Deboki, Nayak, Sunita, Dey, Tuli, Kundu, Subhas C.
Format: Article
Language:English
Subjects:
631/80/83
692/308
Adhesion
Alloys
Animals
Bone implants
Cell Adhesion
Cell adhesion & migration
Cell Communication
Cell Culture Techniques
Cell Line
Cell Proliferation
Cell spreading
Cell Survival
Coculture Techniques
Cytokines
Cytoskeleton - metabolism
Dental prosthetics
Dental restorative materials
Fibroins - chemistry
Humanities and Social Sciences
Macrophages
Macrophages - metabolism
Mice
Mineralization
multidisciplinary
Osseointegration
Osteoblastogenesis
Osteoblasts - metabolism
Osteogenesis
Science
Silk
Silk - chemistry
Surface Properties
Titanium
Titanium - chemistry
Transplants & implants
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Summary:The titanium and its alloys are used as orthopedic dental implants due to their mechanical and bio-inert properties. The bare metal implants are not the ultimate answer for better osteogenesis and implant integration. Physical and chemical modifications are carried out to achieve the goal of improved adhesion and differentiation of the osteoblast. In this work, the silk fibroins from both mulberry and non-mulberry sources are used for surface modification. Silk fibroins are immobilized on titanium surface to facilitate the initial cell adhesion followed by improved cell spreading and better mineralization in order to achieve enhanced osseointegration. The immunological responses along with the effect of cytokines on osteoblast adhesion and function are investigated. The non-mulberry fibroin performs better in the context of the cell adherence and differentiation, which lead to better mineralization. The results indicate that the silk fibroin from non-mulberry source can be used for better osteogenesis on orthopedic implants.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep04745