The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings

• Published in: Acta pharmacologica Sinica 2011-04, Vol.32 (4), p.456-464
• Main Authors: Sun, Yan, Tang, Chao-shu, Jin, Hong-fang, Du, Jun-bao
• Format: Article
• Language: English
• Subjects: Animals; Aorta - drug effects; Aorta - physiology; ATP敏感性钾通道; Base Sequence; Biomedical and Life Sciences; Biomedicine; DNA Primers; Dose-Response Relationship, Drug; Hydrogen Sulfide - pharmacology; Immunohistochemistry; Immunology; In Vitro Techniques; Internal Medicine; KATP通道; Male; Medical Microbiology; Original; original-article; Pharmacology/Toxicology; Pulmonary Artery - drug effects; Pulmonary Artery - physiology; Rats; Rats, Wistar; Vaccine; Vasodilator Agents - pharmacology; 主动脉; 大鼠; 硫化氢; 离体; 肺动脉; 血管舒张
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1038/aps.2011.9

Aim： To compare the vasorelaxing effects of hydrogen sulfide （H2S） on isolated aortic and pulmonary artery rings and to determine their action mechanisms. Methods： H2S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95%O2 and 5% COg was analyzed. The expression of cystathinonine gamma-lyase （CSE）, cystathionine beta synthase （CBS）, 3-mercaptopyruvate sulfurtransferase （3MST）, SUR2B and Kir6.1 was examined. Results： NariS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NariS dilated aortic rings to a greater extent （16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 pmol/L, respectively） than pulmonary artery rings （10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 pmol/L, respectively）. The EC50 of the vasorelaxant effect for aortic rings was 152.17 μmol/L, whereas the EC50 for pulmonary artery rings was 233.65 μmol/L. The vasorelaxing effect of H2S was markedly blocked b y cellular and mitochondrial membrane KATP channel block-ers in aortic rings （P〈0.01）. In contrast, only the cellular membrane KATp channel blocker inhibited H2S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase （CSE） but not cystathionine beta synthase （CBS） expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase （3MST） mRNA was lower in aortic rings than in pulmonary artery rings. Conclusion： The vasorelaxing effect of H2S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the KATp channel subunit SUR2B.