Soluble ST2 protein inhibits LPS stimulation on monocyte-derived dendritic cells

ST2 protein is a soluble splicing variant of ST2L protein, which is the receptor for interleukin-33 （IL-33）. Previously, we reported that soluble ST2 suppressed the signal transduction of lipopolysaccharide （LPS） and cytokine production in monocytic cells. To investigate whether or not this inhibito...

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Bibliographic Details
Published in:Cellular & molecular immunology 2012-09, Vol.9 (5), p.399-409
Main Authors: Nagata, Akihisa, Takezako, Naoki, Tamemoto, Hiroyuki, Ohto-Ozaki, Hiromi, Ohta, Satoshi, Tominaga, Shin-ichi, Yanagisawa, Ken
Format: Article
Language:English
Subjects:
Adaptive immunity
Antibodies
Biomedical and Life Sciences
Biomedicine
Cell Differentiation
Cell proliferation
Cells, Cultured
Cytokines - biosynthesis
Cytokines - immunology
Dendritic cells
Dendritic Cells - cytology
Dendritic Cells - metabolism
Humans
Immunology
Interleukin-1 Receptor-Like 1 Protein
Interleukin-33
Interleukins - metabolism
Lipopolysaccharides
Lipopolysaccharides - pharmacology
LPS
Lymphocytes T
Medical Microbiology
Microbiology
Monocytes
Monocytes - cytology
Monocytes - metabolism
Phenotype
Protein Transport
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
research-article
Signal Transduction
Splicing
ST2
Vaccine
单核细胞
可溶性
树突状细胞
细胞因子
细胞来源
蛋白质
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Summary:ST2 protein is a soluble splicing variant of ST2L protein, which is the receptor for interleukin-33 （IL-33）. Previously, we reported that soluble ST2 suppressed the signal transduction of lipopolysaccharide （LPS） and cytokine production in monocytic cells. To investigate whether or not this inhibitory effect occurs in dendritic cells, which are the key players in innate and adaptive immunity, human monocyte-derived dendritic cells were pre-treated with soluble ST2 protein before LPS stimulation. Although soluble ST2 did not attenuate the LPS-induced maturation of dendritic cells, pre-treatment with soluble ST2 suppressed cytokine production and inhibited LPS signaling. Moreover, the proliferation of naive T cells was inhibited significantly by soluble ST2 pre-treatment. IL-33 had little effect on the cytokine production of immature monocyte-derived dendritic cells. Furthermore, soluble ST2 protein was internalized into dendritic cells, suggesting that soluble ST2 protein acts by a noncanonical mechanism other than the sequestration of IL-33.
ISSN:1672-7681
2042-0226
DOI:10.1038/cmi.2012.29