Effect of acetaminophen (paracetamol) on human osteosarcoma cell line MG63

Aim: To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63. Methods: Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, an...

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Bibliographic Details
Published in:Acta pharmacologica Sinica 2010-11, Vol.31 (11), p.1495-1499
Main Authors: Díaz-Rodríguez, Lourdes, García-Martínez, Olga, Arroyo-Morales, Manuel, Rubio-Ruiz, Belén, Ruiz, Concepción
Format: Article
Language:English
Subjects:
Acetaminophen - pharmacology
Anti-Inflammatory Agents, Non-Steroidal - pharmacology
Antigen Presentation - drug effects
B7-1 Antigen - biosynthesis
B7-2 Antigen - biosynthesis
Biomedical and Life Sciences
Biomedicine
Cell Culture Techniques
Cell Line, Tumor
Cell Proliferation - drug effects
Dose-Response Relationship, Drug
Flow Cytometry
HLA-DR Antigens - biosynthesis
Humans
Immunology
Internal Medicine
Medical Microbiology
Original
original-article
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - immunology
Osteoblasts - physiology
Osteocalcin - biosynthesis
Phagocytosis - drug effects
Phagocytosis - immunology
Pharmacology/Toxicology
T-Lymphocytes - immunology
Vaccine
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Summary:Aim: To examine the effects of acetaminophen (paracetamol), a nonsteroidal anti-inflammatory drug (NSAID), on different cellular and functional parameters of the human osteosarcoma cell line MG63. Methods: Flow cytometry was used to study proliferation, antigenic profile, and phagocytic activity, and radioimmunoassay was used to determine osteocalcin synthesis as a cell differentiation marker. Results: Short-term treatment with therapeutic doses of paracetamol(5 or 25 μmol/L) reduced cell proliferation, osteocalcin synthesis, and phagocyte activity, and increased the expression of antigens involved in antigen presentation to T lymphocytes (CD80, CD86, HLA-DR). Conclusion: These findings suggest that paracetamol activates the osteoblast, inducing its immunogenic action to the detriment of its bone formation capacity.
ISSN:1671-4083
1745-7254
DOI:10.1038/aps.2010.129