The role of bone marrow derived-mesenchymal stem cells in attenuation of kidney function in rats with diabetic nephropathy

Stem cell therapy holds a great promise for the repair of injured tissues and organs, including the kidney. We studied the effect of mesenchymal stem cells (MSC) on experimental diabetic nephropathy (DN) in rats and the possible paracrine signals that mediate their action. Rats were divided into con...

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Published in:Diabetology and metabolic syndrome 2014-03, Vol.6 (1), p.34-34, Article 34
Main Authors: Abdel Aziz, Mohamed Talaat, Wassef, Mohamed Abdel Aziz, Ahmed, Hanan Hosni, Rashed, Laila, Mahfouz, Soheir, Aly, Mayssa Ibrahim, Hussein, Rania Elsayed, Abdelaziz, Mai
Format: Article
Language:English
Subjects:
Albumin
Bone marrow
Care and treatment
Diabetes
Gene expression
Genes
Histochemistry
Medicine
Mortality
Non-Hodgkin's lymphomas
Rodents
Stem cells
Transplantation
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Summary:Stem cell therapy holds a great promise for the repair of injured tissues and organs, including the kidney. We studied the effect of mesenchymal stem cells (MSC) on experimental diabetic nephropathy (DN) in rats and the possible paracrine signals that mediate their action. Rats were divided into controls, DN rats, DN rats receiving MSCs. MSCs were given in a dose of (106cells) by intravenous injection. After 4 weeks, 24 h urinary albumin, serum urea and creatinine concentrations, transforming growth factor β (TGF β), tumor necrosis factor α (TNFα), B-cell lymphoma 2 (bcl2) and Bax gene expression and vascular endothelial growth factor (VEGF) were assessed. Histopathology staining was performed. MSC therapy significantly improved 24 h urinary albumin, serum urea and creatinine concentrations, increased angiogenic growth factor VEGF, and anti-apoptotic protein bcl2 while decreased the pro-inflammatory TNF-α, fibrogenic growth factor TGF β, and pro-apoptotic protein Bax. The histopathology examination showed patchy areas of minimal necrosis and degeneration in renal tubules.
ISSN:1758-5996
1758-5996
DOI:10.1186/1758-5996-6-34