Mast cell degranulation induced by chlorogenic acid

• Published in: Acta pharmacologica Sinica 2010-07, Vol.31 (7), p.849-854
• Main Authors: Huang, Fang-hua, Zhang, Xin-yue, Zhang, Lu-yong, Li, Qin, Ni, Bin, Zheng, Xiao-liang, Chen, Ai-jun
• Format: Article
• Language: English
• Subjects: Anaphylaxis - chemically induced; Animals; beta-N-Acetylhexosaminidases - metabolism; Biomedical and Life Sciences; Biomedicine; Cell Degranulation - drug effects; Cell Line, Tumor; Chlorogenic Acid - administration & dosage; Chlorogenic Acid - toxicity; Dose-Response Relationship, Drug; Flow Cytometry; Guinea Pigs; Immunology; Internal Medicine; Male; Mast Cells - metabolism; Mast Cells - physiology; Medical Microbiology; Microscopy, Electron, Transmission; Original; original-article; Peritoneum - cytology; Peritoneum - metabolism; Pharmacology/Toxicology; Rats; Time Factors; Vaccine; 时间依赖性; 流式细胞仪分析; 绿原酸; 肥大细胞; 过敏性反应; 透射电子显微镜
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1038/aps.2010.63

Aim： To investigate the mechanism of chlorogenic acid （CA）-induced anaphylactoid reactions. Methods： Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index （DI） was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and β-hexosaminidase release. Results： CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulatJon of peritoneal mast cells Jn guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control （P〉0.05）. Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner （P〈0.01）. Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. Fluorescent microscopy and flow cytometric analysis showed that CA induced concentration-dependent translocation of phosphatidylserine in RBL- 2H3 cells. β-hexosaminidase release in RBL-2H3 cells was significantly increased after incubation with I mmol/L CA for 60 min and 5 mmol/L CA for 30 min （P〈0.01）. Conclusion： CA induces degranulation of peritoneal mast cells and RBL-2H3 cells in guinea pigs, which might be one of the mechanisms of the generation of anaphylactoid reactions induced by CA.