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Mast cell degranulation induced by chlorogenic acid

Aim: To investigate the mechanism of chlorogenic acid (CA)-induced anaphylactoid reactions. Methods: Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also ob...

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Published in:Acta pharmacologica Sinica 2010-07, Vol.31 (7), p.849-854
Main Authors: Huang, Fang-hua, Zhang, Xin-yue, Zhang, Lu-yong, Li, Qin, Ni, Bin, Zheng, Xiao-liang, Chen, Ai-jun
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description Aim: To investigate the mechanism of chlorogenic acid (CA)-induced anaphylactoid reactions. Methods: Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and β-hexosaminidase release. Results: CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulatJon of peritoneal mast cells Jn guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control (P〉0.05). Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner (P〈0.01). Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. Fluorescent microscopy and flow cytometric analysis showed that CA induced concentration-dependent translocation of phosphatidylserine in RBL- 2H3 cells. β-hexosaminidase release in RBL-2H3 cells was significantly increased after incubation with I mmol/L CA for 60 min and 5 mmol/L CA for 30 min (P〈0.01). Conclusion: CA induces degranulation of peritoneal mast cells and RBL-2H3 cells in guinea pigs, which might be one of the mechanisms of the generation of anaphylactoid reactions induced by CA.
doi_str_mv 10.1038/aps.2010.63
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Methods: Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and β-hexosaminidase release. Results: CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulatJon of peritoneal mast cells Jn guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control (P〉0.05). Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner (P〈0.01). Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. Fluorescent microscopy and flow cytometric analysis showed that CA induced concentration-dependent translocation of phosphatidylserine in RBL- 2H3 cells. β-hexosaminidase release in RBL-2H3 cells was significantly increased after incubation with I mmol/L CA for 60 min and 5 mmol/L CA for 30 min (P〈0.01). Conclusion: CA induces degranulation of peritoneal mast cells and RBL-2H3 cells in guinea pigs, which might be one of the mechanisms of the generation of anaphylactoid reactions induced by CA.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><identifier>DOI: 10.1038/aps.2010.63</identifier><identifier>PMID: 20581858</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Anaphylaxis - chemically induced ; Animals ; beta-N-Acetylhexosaminidases - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Cell Degranulation - drug effects ; Cell Line, Tumor ; Chlorogenic Acid - administration &amp; dosage ; Chlorogenic Acid - toxicity ; Dose-Response Relationship, Drug ; Flow Cytometry ; Guinea Pigs ; Immunology ; Internal Medicine ; Male ; Mast Cells - metabolism ; Mast Cells - physiology ; Medical Microbiology ; Microscopy, Electron, Transmission ; Original ; original-article ; Peritoneum - cytology ; Peritoneum - metabolism ; Pharmacology/Toxicology ; Rats ; Time Factors ; Vaccine ; 时间依赖性 ; 流式细胞仪分析 ; 绿原酸 ; 肥大细胞 ; 过敏性反应 ; 透射电子显微镜</subject><ispartof>Acta pharmacologica Sinica, 2010-07, Vol.31 (7), p.849-854</ispartof><rights>CPS and SIMM 2010</rights><rights>Copyright Nature Publishing Group Jul 2010</rights><rights>Copyright © 2010 CPS and SIMM 2010 CPS and SIMM</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c527t-f5a59f458681ed2767de118c152cf5c274f2aa2dd730fba7320d94f5a10e54513</citedby><cites>FETCH-LOGICAL-c527t-f5a59f458681ed2767de118c152cf5c274f2aa2dd730fba7320d94f5a10e54513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007731/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007731/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20581858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Fang-hua</creatorcontrib><creatorcontrib>Zhang, Xin-yue</creatorcontrib><creatorcontrib>Zhang, Lu-yong</creatorcontrib><creatorcontrib>Li, Qin</creatorcontrib><creatorcontrib>Ni, Bin</creatorcontrib><creatorcontrib>Zheng, Xiao-liang</creatorcontrib><creatorcontrib>Chen, Ai-jun</creatorcontrib><title>Mast cell degranulation induced by chlorogenic acid</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacol Sin</addtitle><addtitle>Acta Pharmacologica Sinica</addtitle><description>Aim: To investigate the mechanism of chlorogenic acid (CA)-induced anaphylactoid reactions. Methods: Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and β-hexosaminidase release. Results: CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulatJon of peritoneal mast cells Jn guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control (P〉0.05). Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner (P〈0.01). Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. 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Methods: Degranulation of peritoneal mast cells was assayed by using alcian blue staining in guinea pigs, and the degranulation index (DI) was calculated. CA-induced degranulation of RBL-2H3 cells was also observed and assayed using light microscopy, transmission electron microscopy, flow cytometry, and β-hexosaminidase release. Results: CA 0.2, 1.0, and 5.0 mmol/L was able to promote degranulatJon of peritoneal mast cells Jn guinea pigs in vitro, but it did not increase the degranulation of peritoneal mast cells in CA-sensitized guinea pigs compared with control (P〉0.05). Treatment with CA 0.2, 1.0, and 5.0 mmol/L for 30, 60, and 120 min induced degranulation in RBL-2H3 cells in a dose- and time-dependent manner (P〈0.01). Under transmission electron microscope typical characteristics of degranulation, including migration of granular vesicles toward the plasma membrane and integration combined with exocytosis, were observed, after CA or C48/80 treatment. Fluorescent microscopy and flow cytometric analysis showed that CA induced concentration-dependent translocation of phosphatidylserine in RBL- 2H3 cells. β-hexosaminidase release in RBL-2H3 cells was significantly increased after incubation with I mmol/L CA for 60 min and 5 mmol/L CA for 30 min (P〈0.01). Conclusion: CA induces degranulation of peritoneal mast cells and RBL-2H3 cells in guinea pigs, which might be one of the mechanisms of the generation of anaphylactoid reactions induced by CA.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>20581858</pmid><doi>10.1038/aps.2010.63</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects Anaphylaxis - chemically induced
Animals
beta-N-Acetylhexosaminidases - metabolism
Biomedical and Life Sciences
Biomedicine
Cell Degranulation - drug effects
Cell Line, Tumor
Chlorogenic Acid - administration & dosage
Chlorogenic Acid - toxicity
Dose-Response Relationship, Drug
Flow Cytometry
Guinea Pigs
Immunology
Internal Medicine
Male
Mast Cells - metabolism
Mast Cells - physiology
Medical Microbiology
Microscopy, Electron, Transmission
Original
original-article
Peritoneum - cytology
Peritoneum - metabolism
Pharmacology/Toxicology
Rats
Time Factors
Vaccine
时间依赖性
流式细胞仪分析
绿原酸
肥大细胞
过敏性反应
透射电子显微镜
title Mast cell degranulation induced by chlorogenic acid
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