Imaging the Lipid-Phase-Dependent Pore Formation of Equinatoxin II in Droplet Interface Bilayers

Using phase-separated droplet interface bilayers, we observe membrane binding and pore formation of a eukaryotic cytolysin, Equinatoxin II (EqtII). EqtII activity is known to depend on the presence of sphingomyelin in the target membrane and is enhanced by lipid phase separation. By imaging the ioni...

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Bibliographic Details
Published in:Biophysical journal 2014-04, Vol.106 (8), p.1630-1637
Main Authors: Rojko, N., Cronin, B., Danial, J.S.H., Baker, M.A.B., Anderluh, G., Wallace, M.I.
Format: Article
Language:English
Subjects:
Animals
Binding sites
Chickens
Cnidarian Venoms - chemistry
Eukaryotes
Lipid Bilayers - chemistry
Lipid Droplets - chemistry
Lipids
Membranes
Molecular Imaging - methods
Porosity
Sheep
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Summary:Using phase-separated droplet interface bilayers, we observe membrane binding and pore formation of a eukaryotic cytolysin, Equinatoxin II (EqtII). EqtII activity is known to depend on the presence of sphingomyelin in the target membrane and is enhanced by lipid phase separation. By imaging the ionic flux through individual pores in vitro, we observe that EqtII pores form predominantly within the liquid-disordered phase. We observe preferential binding of labeled EqtII at liquid-ordered/liquid-disordered domain boundaries before it accumulates in the liquid-disordered phase.
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2013.11.4507