Anopheles stephensi salivary glands bear receptors for region I of the circumsporozoite protein of Plasmodium falciparum

In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1997-12, Vol.90 (1), p.33-41
Main Authors: Sidjanski, Sacha P, Vanderberg, Jerome P, Sinnis, Photini
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Anopheles - metabolism
Anopheles stephensi
binding
Binding Sites
Circumsporozoite protein
circumsporozoite proteins
Female
inhibition
Malaria
Male
Molecular Sequence Data
Mosquito
Organ Specificity
Peptide Fragments - pharmacology
peptides
Plasmodium
Plasmodium falciparum
Plasmodium falciparum - chemistry
Protozoan Proteins - chemistry
Protozoan Proteins - metabolism
receptors
Recombinant Proteins - metabolism
Salivary glands
Salivary Glands - metabolism
Sporozoite
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Summary:In the mosquito, Plasmodium sporozoites rupture from oocysts found on the midgut wall, circulate in the hemolymph and invade salivary glands where they wait to be injected into a vertebrate host during a bloodmeal. The mechanisms by which sporozoites specifically attach to and invade salivary glands are not known but evidence suggests that it is a receptor-mediated process. Here we show that the major surface protein of sporozoites, the circumsporozoite protein (CS), binds preferentially to salivary glands when compared to other organs exposed to the circulating hemolymph. In addition, we show that a peptide encompassing region I, a highly conserved sequence found in all rodent and primate Plasmodium CS proteins, inhibits binding of CS to mosquito salivary glands.
ISSN:0166-6851
1872-9428
DOI:10.1016/S0166-6851(97)00124-2