Efficacy of subantimicrobial-dose doxycycline against nitrosative stress in chronic periodontitis

• Published in: Acta pharmacologica Sinica 2013-02, Vol.34 (2), p.247-254
• Main Authors: Pârvu, Alina Elena, Alb, Sandu Florin, Crăciun, Alexandra, Taulescu, Marian Aurel
• Format: Article
• Language: English
• Subjects: Adult; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - therapeutic use; Biomedical and Life Sciences; Biomedicine; Chronic Periodontitis - blood; Chronic Periodontitis - drug therapy; Chronic Periodontitis - pathology; Dental Scaling - methods; Doxycycline - administration & dosage; Doxycycline - therapeutic use; Female; Gingiva - pathology; Humans; Immunohistochemistry; Immunology; Internal Medicine; Male; Medical Microbiology; Middle Aged; Nitrates - blood; Nitric Oxide - blood; Nitric Oxide Synthase - analysis; Nitrites - blood; Original; original-article; Pharmacology/Toxicology; Root Planing - methods; Tyrosine - analogs & derivatives; Tyrosine - analysis; Vaccine; 剂量; 压力参数; 反硝化; 强力霉素; 慢性; 治疗; 牙周炎; 诱导型一氧化氮合酶
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1038/aps.2012.129

Aim： To evaluate the effectiveness of subantimicrobial-dose doxycycline （SDD） as an adjunct to scaling and root planing （SRP） treatment against the nitrosative stress of moderate to advanced chronic periodontitis. Methods： Adults with untreated chronic periodontitis （n=174） were randomly administered SRP＋SDD （n=87） （20 mg of doxycycline twice daily） or SRP＋placebo （n=87） treatment for 3 months. At baseline and after 3 months, the probing depths （PD）, bleeding on probing （BOP） and clinical attachment level （CAL） were measured, and a gingivomucosal biopsy was collected to assay the induction of nitric oxide synthase （iNOS） and 3-nitrotyrosine （3NT）, and blood was collected to assay for total nitrites and nitrates （NOx） and 3NT. Results： Compared to baseline, at the completion of treatment, significant decreases in the levels of tissue iNOS and 3NT and serum NOx and 3NT were observed in both groups. SRP＋SDD yielded a greater reduction in the gingivomucosal and serum nitrosative stress markers than did SRP＋placebo. PD, BOP, and CAL reduction were correlated with the nitrosative stress parameters. Conclusion： On a short-term basis, SDD therapy may be used as an adjunct to SRP treatment against nitrosative stress in moderate to advanced chronic periodontitis.