Plasma levels of advanced glycation endproducts are associated with type 1 diabetes and coronary artery calcification

• Published in: Cardiovascular diabetology 2013-10, Vol.12 (1), p.149-149, Article 149
• Main Authors: van Eupen, Marcelle G A, Schram, Miranda T, Colhoun, Helen M, Scheijen, Jean L J M, Stehouwer, Coen D A, Schalkwijk, Casper G
• Format: Article
• Language: English
• Subjects: Adult; Advanced glycosylation end products; Arginine - analogs & derivatives; Arginine - blood; Biomarkers - blood; C-Reactive Protein - metabolism; Cardiovascular disease; Case-Control Studies; Chromatography, High Pressure Liquid; Coronary Artery Disease - blood; Coronary Artery Disease - diagnostic imaging; Coronary Vessels - diagnostic imaging; Diabetes; Diabetes Mellitus, Type 1 - blood; Female; Glycation End Products, Advanced - blood; Humans; Lysine - analogs & derivatives; Lysine - blood; Male; Middle Aged; Original Investigation; Plasma; Pyrimidines - blood; Studies; Tomography, X-Ray Computed; Vascular Calcification - blood; Vascular Calcification - diagnostic imaging; Vascular Cell Adhesion Molecule-1 - blood; Veins & arteries; von Willebrand Factor - metabolism
• ISSN: 1475-2840; 1475-2840
• DOI: 10.1186/1475-2840-12-149

Advanced glycation endproducts (AGEs) may play a role in the development of coronary artery calcification (CAC) in type 1 diabetes (T1DM). We studied plasma AGEs in association with T1DM and CAC, and whether or not the latter association could be explained by low-grade inflammation (LGI) or endothelial dysfunction (ED). We studied 165 individuals with and 169 without T1DM. CAC was quantified in a CAC score based on CT-scanning. Plasma levels of protein-bound pentosidine, Nϵ-(carboxymethyl)lysine (CML) and Nϵ-(carboxyethyl)lysine (CEL) were measured with HPLC/UPLC with fluorescence detection or tandem-mass spectrometry. Tetrahydropyrimidine (THP) was measured with ELISA, as were HsCRP, and sVCAM-1 and vWF, as markers for LGI and ED, respectively. Associations were analyzed with ANCOVA and adjusted for age, sex, BMI, waist-to-hip ratio, smoking, blood pressure, lipid profile, eGFR and T1DM. Individuals with T1DM had higher plasma levels of pentosidine, CML and THP compared with controls; means (95% CI) were 0.69 (0.65-0.73) vs. 0.51 (0.48-0.54) nmol/mmol LYS, p