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Increase in hippocampal water diffusion and volume during experimental pneumococcal meningitis is aggravated by bacteremia
The hippocampus undergoes apoptosis in experimental pneumococcal meningitis leading to neurofunctional deficits in learning and memory function. The aim of the present study was 1) to investigate hippocampal apparent diffusion coefficient (ADC) and volume with MRI during the course of experimental p...
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| Published in: | BMC infectious diseases 2014-05, Vol.14 (1), p.240-240, Article 240 |
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| creator | Holler, Jon G Brandt, Christian T Leib, Stephen L Rowland, Ian J Østergaard, Christian |
| description | The hippocampus undergoes apoptosis in experimental pneumococcal meningitis leading to neurofunctional deficits in learning and memory function. The aim of the present study was 1) to investigate hippocampal apparent diffusion coefficient (ADC) and volume with MRI during the course of experimental pneumococcal meningitis, 2) to explore the influence of accompanying bacteremia on hippocampal water distribution and volume, 3) and to correlate these findings to the extent of apoptosis in the hippocampus.
Experimental meningitis in rats was induced by intracisternal injection of live pneumococci. The study comprised of four experimental groups. I. Uninfected controls (n = 8); II. Meningitis (n = 11); III. Meningitis with early onset bacteremia by additional i.v. injection of live pneumococci (n = 10); IV. Meningitis with attenuated bacteremia by treatment with serotype-specific anti-pneumococcal antibodies (n = 14). T2 and diffusion weighted MR images were used to analyze changes in hippocampus volume and water diffusion (ADC). The results were correlated to ADC of the cortex, to ventricular volume, and to the extent of hippocampal apoptosis.
Both ADC and the volume of hippocampus were significantly increased in meningitis rats compared to uninfected controls (Kruskal-Wallis test, p = 0.0001, Dunns Post Test, p |
| doi_str_mv | 10.1186/1471-2334-14-240 |
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Experimental meningitis in rats was induced by intracisternal injection of live pneumococci. The study comprised of four experimental groups. I. Uninfected controls (n = 8); II. Meningitis (n = 11); III. Meningitis with early onset bacteremia by additional i.v. injection of live pneumococci (n = 10); IV. Meningitis with attenuated bacteremia by treatment with serotype-specific anti-pneumococcal antibodies (n = 14). T2 and diffusion weighted MR images were used to analyze changes in hippocampus volume and water diffusion (ADC). The results were correlated to ADC of the cortex, to ventricular volume, and to the extent of hippocampal apoptosis.
Both ADC and the volume of hippocampus were significantly increased in meningitis rats compared to uninfected controls (Kruskal-Wallis test, p = 0.0001, Dunns Post Test, p < 0.05), and were significantly increased in meningitis rats with an early onset bacteremia as compared to meningitis rats with attenuated bacteremia (p < 0.05). Hippocampal ADC and the volume and size of brain ventricles were positively correlated (Spearman Rank, p < 0.05), whereas no association was found between ADC or volume and the extent of apoptosis (p > 0.05).
In experimental meningitis increase in volume and water diffusion of the hippocampus are significantly associated with accompanying bacteremia.</description><identifier>ISSN: 1471-2334</identifier><identifier>EISSN: 1471-2334</identifier><identifier>DOI: 10.1186/1471-2334-14-240</identifier><identifier>PMID: 24886045</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Animals ; Apoptosis ; Bacteremia - metabolism ; Bacteremia - microbiology ; Bacteremia - pathology ; Brain ; Cerebral Cortex - metabolism ; Cerebral Ventricles - pathology ; Diffusion ; Disease Models, Animal ; Hippocampus - metabolism ; Hippocampus - pathology ; Magnetic Resonance Imaging ; Male ; Meningitis ; Meningitis, Pneumococcal - metabolism ; Meningitis, Pneumococcal - pathology ; Rats ; Rats, Wistar ; Rodents ; Streptococcus pneumoniae ; Studies ; Water - metabolism</subject><ispartof>BMC infectious diseases, 2014-05, Vol.14 (1), p.240-240, Article 240</ispartof><rights>COPYRIGHT 2014 BioMed Central Ltd.</rights><rights>2014 Holler et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.</rights><rights>Copyright © 2014 Holler et al.; licensee BioMed Central Ltd. 2014 Holler et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b652t-a67bab0c3dbdb0b47063e97b29ec00d4a219460524f7ef08e5609a1f9490f62c3</citedby><cites>FETCH-LOGICAL-b652t-a67bab0c3dbdb0b47063e97b29ec00d4a219460524f7ef08e5609a1f9490f62c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4016615/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1522968745?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24886045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holler, Jon G</creatorcontrib><creatorcontrib>Brandt, Christian T</creatorcontrib><creatorcontrib>Leib, Stephen L</creatorcontrib><creatorcontrib>Rowland, Ian J</creatorcontrib><creatorcontrib>Østergaard, Christian</creatorcontrib><title>Increase in hippocampal water diffusion and volume during experimental pneumococcal meningitis is aggravated by bacteremia</title><title>BMC infectious diseases</title><addtitle>BMC Infect Dis</addtitle><description>The hippocampus undergoes apoptosis in experimental pneumococcal meningitis leading to neurofunctional deficits in learning and memory function. The aim of the present study was 1) to investigate hippocampal apparent diffusion coefficient (ADC) and volume with MRI during the course of experimental pneumococcal meningitis, 2) to explore the influence of accompanying bacteremia on hippocampal water distribution and volume, 3) and to correlate these findings to the extent of apoptosis in the hippocampus.
Experimental meningitis in rats was induced by intracisternal injection of live pneumococci. The study comprised of four experimental groups. I. Uninfected controls (n = 8); II. Meningitis (n = 11); III. Meningitis with early onset bacteremia by additional i.v. injection of live pneumococci (n = 10); IV. Meningitis with attenuated bacteremia by treatment with serotype-specific anti-pneumococcal antibodies (n = 14). T2 and diffusion weighted MR images were used to analyze changes in hippocampus volume and water diffusion (ADC). The results were correlated to ADC of the cortex, to ventricular volume, and to the extent of hippocampal apoptosis.
Both ADC and the volume of hippocampus were significantly increased in meningitis rats compared to uninfected controls (Kruskal-Wallis test, p = 0.0001, Dunns Post Test, p < 0.05), and were significantly increased in meningitis rats with an early onset bacteremia as compared to meningitis rats with attenuated bacteremia (p < 0.05). Hippocampal ADC and the volume and size of brain ventricles were positively correlated (Spearman Rank, p < 0.05), whereas no association was found between ADC or volume and the extent of apoptosis (p > 0.05).
In experimental meningitis increase in volume and water diffusion of the hippocampus are significantly associated with accompanying bacteremia.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Bacteremia - metabolism</subject><subject>Bacteremia - microbiology</subject><subject>Bacteremia - pathology</subject><subject>Brain</subject><subject>Cerebral Cortex - metabolism</subject><subject>Cerebral Ventricles - pathology</subject><subject>Diffusion</subject><subject>Disease Models, Animal</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - pathology</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Meningitis</subject><subject>Meningitis, Pneumococcal - metabolism</subject><subject>Meningitis, Pneumococcal - pathology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Streptococcus pneumoniae</subject><subject>Studies</subject><subject>Water - metabolism</subject><issn>1471-2334</issn><issn>1471-2334</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNqNkk1r3DAQhk1padJt7z0VQS_pwakky7J9KYSlHwuBQL-uQpbHjoItuZK9TfrrO8um27ikECSQNHrm1fCOkuQlo6eMlfItEwVLeZaJlImUC_ooOT6EHt_ZHyXPYryilBUlr54mR1yUpaQiP05-bZwJoCMQ68ilHUdv9DDqnvzUEwTS2Lado_WOaNeQre_nAUgzB-s6AtcjBDuAmxAfHcyDN94YPGAMATvZSHDqrgt6i3INqW9IrQ0Kw2D18-RJq_sIL27XVfLtw_uv60_p-cXHzfrsPK1lzqdUy6LWNTVZUzc1rUVBZQZVUfMKDKWN0JxVQtKci7aAlpaQS1pp1laioq3kJlsl7_a641wP0BgsOOhejVi7DjfKa6uWN85eqs5vlaBMSpajwHovUFv_H4HljfGD2nmvdt7jTmFrUOXktozgf8wQJzXYaKDvtQM_R4UPiTJjmcwegvJK0CIXiL7-B73yc3DoJ1KcV7IsRP6X6nQPyrrWY51mJ6rO8qzKi5yhq6vk9B4KR4PtMt5BazG-SHizSEBmguup03OMavPl88PZi-9Llu5ZE3yMAdqD14yq3ce_z91Xd5t8SPjz07PfhYD9TQ</recordid><startdate>20140506</startdate><enddate>20140506</enddate><creator>Holler, Jon G</creator><creator>Brandt, Christian T</creator><creator>Leib, Stephen L</creator><creator>Rowland, Ian J</creator><creator>Østergaard, Christian</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T2</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20140506</creationdate><title>Increase in hippocampal water diffusion and volume during experimental pneumococcal meningitis is aggravated by bacteremia</title><author>Holler, Jon G ; 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The aim of the present study was 1) to investigate hippocampal apparent diffusion coefficient (ADC) and volume with MRI during the course of experimental pneumococcal meningitis, 2) to explore the influence of accompanying bacteremia on hippocampal water distribution and volume, 3) and to correlate these findings to the extent of apoptosis in the hippocampus.
Experimental meningitis in rats was induced by intracisternal injection of live pneumococci. The study comprised of four experimental groups. I. Uninfected controls (n = 8); II. Meningitis (n = 11); III. Meningitis with early onset bacteremia by additional i.v. injection of live pneumococci (n = 10); IV. Meningitis with attenuated bacteremia by treatment with serotype-specific anti-pneumococcal antibodies (n = 14). T2 and diffusion weighted MR images were used to analyze changes in hippocampus volume and water diffusion (ADC). The results were correlated to ADC of the cortex, to ventricular volume, and to the extent of hippocampal apoptosis.
Both ADC and the volume of hippocampus were significantly increased in meningitis rats compared to uninfected controls (Kruskal-Wallis test, p = 0.0001, Dunns Post Test, p < 0.05), and were significantly increased in meningitis rats with an early onset bacteremia as compared to meningitis rats with attenuated bacteremia (p < 0.05). Hippocampal ADC and the volume and size of brain ventricles were positively correlated (Spearman Rank, p < 0.05), whereas no association was found between ADC or volume and the extent of apoptosis (p > 0.05).
In experimental meningitis increase in volume and water diffusion of the hippocampus are significantly associated with accompanying bacteremia.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>24886045</pmid><doi>10.1186/1471-2334-14-240</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Bacteremia - metabolism Bacteremia - microbiology Bacteremia - pathology Brain Cerebral Cortex - metabolism Cerebral Ventricles - pathology Diffusion Disease Models, Animal Hippocampus - metabolism Hippocampus - pathology Magnetic Resonance Imaging Male Meningitis Meningitis, Pneumococcal - metabolism Meningitis, Pneumococcal - pathology Rats Rats, Wistar Rodents Streptococcus pneumoniae Studies Water - metabolism |
| title | Increase in hippocampal water diffusion and volume during experimental pneumococcal meningitis is aggravated by bacteremia |
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