Herbal adaptogens combined with protein fractions from bovine colostrum and hen egg yolk reduce liver TNF-α expression and protein carbonylation in Western diet feeding in rats

BACKGROUND: We examined if a purported anti-inflammatory supplement (AF) abrogated Western-diet (WD)-induced liver pathology in rats. AF contained: 1) protein concentrates from bovine colostrum and avian egg yolk; 2) herbal adaptogens and antioxidants; and 3) acetyl-L-carnitine. METHODS: Nine month-...

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Published in:Nutrition & metabolism 2014-04, Vol.11 (1), p.19-19, Article 19
Main Authors: Mobley, C Brooks, Toedebusch, Ryan G, Lockwood, Christopher M, Heese, Alexander J, Zhu, Conan, Krieger, Anna E, Cruthirds, Clayton L, Hofheins, John C, Company, Joseph M, Wiedmeyer, Charles E, Kim, Dae Y, Booth, Frank W, Roberts, Michael D
Format: Article
Language:English
Subjects:
antioxidant activity
antioxidants
bioinformatics
blood serum
carbohydrate metabolism
cow colostrum
egg yolk
gene expression
genes
hens
ingredients
liver
liver protein
messenger RNA
protein concentrates
protein synthesis
rats
Rattus norvegicus
reverse transcriptase polymerase chain reaction
tumor necrosis factor-alpha
Western diets
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Summary:BACKGROUND: We examined if a purported anti-inflammatory supplement (AF) abrogated Western-diet (WD)-induced liver pathology in rats. AF contained: 1) protein concentrates from bovine colostrum and avian egg yolk; 2) herbal adaptogens and antioxidants; and 3) acetyl-L-carnitine. METHODS: Nine month-old male Brown Norway rats were allowed ad libitum access to WD for 41–43 days and randomly assigned to WD + AF feeding twice daily for the last 31–33 days (n = 8), or WD and water-placebo feeding twice daily for the last 31–33 days (n = 8). Rats fed a low-fat/low-sucrose diet (CTL, n = 6) for 41–43 days and administered a water-placebo twice daily for the last 31–33 days were also studied. Twenty-four hours following the last gavage-feed, liver samples were analyzed for: a) select mRNAs (via RT-PCR) as well as genome-wide mRNA expression patterns (via RNA-seq); b) lipid deposition; and, c) protein carbonyl and total antioxidant capacity (TAC). Serum was also examined for TAC, 8-isoprostane and clinical chemistry markers. RESULTS: WD + AF rats experienced a reduction in liver Tnf-α mRNA (-2.8-fold, p 
ISSN:1743-7075
1743-7075
DOI:10.1186/1743-7075-11-19