Deletion mutant library for investigation of functional outputs of cyclic diguanylate metabolism in Pseudomonas aeruginosa PA14

We constructed a library of in-frame deletion mutants targeting each gene in Pseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. W...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 2014-06, Vol.80 (11), p.3384-3393
Main Authors: Ha, Dae-Gon, Richman, Megan E, O'Toole, George A
Format: Article
Language:English
Subjects:
Bacteria
Biofilms - growth & development
Biotransformation
Cyclic GMP - analogs & derivatives
Cyclic GMP - metabolism
Gene Deletion
Gene Library
Genes
Genetics and Molecular Biology
Genotype & phenotype
Locomotion
Metabolic Networks and Pathways - genetics
Metabolism
Microbiology
Polysaccharides, Bacterial - metabolism
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - metabolism
Pseudomonas aeruginosa - physiology
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Description
Summary:We constructed a library of in-frame deletion mutants targeting each gene in Pseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.00299-14