Correlation between Urothelial Differentiation and Sensory Proteins P2X3, P2X5, TRPV1, and TRPV4 in Normal Urothelium and Papillary Carcinoma of Human Bladder

Terminal differentiation of urothelium is a prerequisite for blood-urine barrier formation and enables normal sensory function of the urinary bladder. In this study, urothelial differentiation of normal human urothelium and of low and high grade papillary urothelial carcinomas was correlated with th...

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Bibliographic Details
Published in:BioMed research international 2014-01, Vol.2014 (2014), p.1-9
Main Authors: Sterle, Igor, Zupančič, Daša, Romih, Rok
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biomedical research
Biopsy
Bladder cancer
Carcinoma, Papillary - metabolism
Cell Differentiation
Excretory system
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Humans
Hypotheses
Microscopy, Electron, Scanning
Microscopy, Fluorescence
Middle Aged
Pathogenesis
Patients
Permeability
Prognosis
Proteins
Receptors, Purinergic P2X3 - metabolism
Receptors, Purinergic P2X5 - metabolism
Rodents
Studies
TRPV Cation Channels - metabolism
Tumors
Urinary Bladder Neoplasms - metabolism
Urothelium
Urothelium - cytology
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Summary:Terminal differentiation of urothelium is a prerequisite for blood-urine barrier formation and enables normal sensory function of the urinary bladder. In this study, urothelial differentiation of normal human urothelium and of low and high grade papillary urothelial carcinomas was correlated with the expression and localization of purinergic receptors (P2X3, and P2X5) and transient receptor potential vanilloid channels (TRPV1, and TRPV4). Western blotting and immunofluorescence of uroplakins together with scanning electron microscopy of urothelial apical surface demonstrated terminal differentiation of normal urothelium, partial differentiation of low grade carcinoma, and poor differentiation of high grade carcinoma. P2X3 was expressed in normal urothelium as well as in low grade carcinoma and in both cases immunolabeling was stronger in the superficial cells. P2X3 expression decreased in high grade carcinoma. P2X5 expression was detected in normal urothelium and in high grade carcinoma, while in low grade carcinoma its expression was diminished. The expression of TRPV1 decreased in low grade and even more in high grade carcinoma when compared with normal urothelium, while TRPV4 expression was unchanged in all samples. Our results suggest that sensory proteins P2X3 and TRPV1 are in correlation with urothelial differentiation, while P2X5 and TRPV4 have unique expression patterns.
ISSN:2314-6133
2314-6141
DOI:10.1155/2014/805236