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Identification and frequency of CCR5Δ32/Δ32 HIV‐resistant cord blood units from Houston area hospitals
Objectives The use of umbilical cord blood (CB) that is genetically resistant to HIV infection has been proposed as a novel stem cell therapy for the treatment of patients with AIDS. These genetically unique CB units (CBUs) should be present in public CB banks at a predicted frequency. Methods The c...
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| Published in: | HIV medicine 2011-09, Vol.12 (8), p.481-486 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c3381-cdb15332d265bbe68171e9ca6778883ff4be1768ff744f08627bc15d94a3a8873 |
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| container_end_page | 486 |
| container_issue | 8 |
| container_start_page | 481 |
| container_title | HIV medicine |
| container_volume | 12 |
| creator | Gonzalez, G Park, SS Chen, DW Armitage, S Shpall, EJ Behringer, RR |
| description | Objectives
The use of umbilical cord blood (CB) that is genetically resistant to HIV infection has been proposed as a novel stem cell therapy for the treatment of patients with AIDS. These genetically unique CB units (CBUs) should be present in public CB banks at a predicted frequency.
Methods
The chemokine (C‐C motif) receptor 5 (CCR5) genotypes of CBUs donated to the M. D. Anderson CB Bank by four Houston area hospitals were determined by polymerase chain reaction (PCR) and DNA sequencing.
Results
The frequency of CCR5Δ32/Δ32 CBUs was consistent with the frequency of the CCR5Δ32 allele in human populations, and was apparently dependent on the ethnic population of the parents of the newborns from whom the CBUs were collected.
Conclusions
Routine genotyping to identify HIV‐resistant CBUs could create a bank of CB‐derived stem/progenitor cells with which to treat HIV infection. |
| doi_str_mv | 10.1111/j.1468-1293.2010.00911.x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4021858</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>893977917</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3381-cdb15332d265bbe68171e9ca6778883ff4be1768ff744f08627bc15d94a3a8873</originalsourceid><addsrcrecordid>eNpVUctqGzEUFSEhTtP8QtGuq7F1pRlJAyUQTBsbDIGQdCs0GimWGY-c0TiNd_2ELPpd_Yh-STR5mEYL3YvO1TnccxDCQMaQzmQ1hpzLDGjJxpSkV0JKgPHjATrZA4cvfZ5RzukIfYpxRQgIVpJjNKLARMFlfoJW89q2vXfe6N6HFuu2xq6z91vbmh0ODk-n18XfP4xOhgvP5j___X7qbPSx122PTehqXDUh1Hjb-j6mv2GNZ2Eb-4GssxovQ9z4XjfxMzpyqdizt3qKbn98v5nOssXV5Xx6scgMYxIyU1dQMEZryouqslyCAFsazYWQUjLn8sqC4NI5keeOSE5FZaCoy1wzLaVgp-j8lXezrda2Nmm_Tjdq0_m17nYqaK8-Iq1fqrvwoHJCQRYyEXx9I-hCMiL2au2jsU2jW5s2U7JkpRAlDFJf_pfaa7z7mwa-vQ788o3d7XEgashRrdQQlxriUkOO6iVH9aiSz6lhz8NMk7o</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>893977917</pqid></control><display><type>article</type><title>Identification and frequency of CCR5Δ32/Δ32 HIV‐resistant cord blood units from Houston area hospitals</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Gonzalez, G ; Park, SS ; Chen, DW ; Armitage, S ; Shpall, EJ ; Behringer, RR</creator><creatorcontrib>Gonzalez, G ; Park, SS ; Chen, DW ; Armitage, S ; Shpall, EJ ; Behringer, RR</creatorcontrib><description>Objectives
The use of umbilical cord blood (CB) that is genetically resistant to HIV infection has been proposed as a novel stem cell therapy for the treatment of patients with AIDS. These genetically unique CB units (CBUs) should be present in public CB banks at a predicted frequency.
Methods
The chemokine (C‐C motif) receptor 5 (CCR5) genotypes of CBUs donated to the M. D. Anderson CB Bank by four Houston area hospitals were determined by polymerase chain reaction (PCR) and DNA sequencing.
Results
The frequency of CCR5Δ32/Δ32 CBUs was consistent with the frequency of the CCR5Δ32 allele in human populations, and was apparently dependent on the ethnic population of the parents of the newborns from whom the CBUs were collected.
Conclusions
Routine genotyping to identify HIV‐resistant CBUs could create a bank of CB‐derived stem/progenitor cells with which to treat HIV infection.</description><identifier>ISSN: 1464-2662</identifier><identifier>EISSN: 1468-1293</identifier><identifier>DOI: 10.1111/j.1468-1293.2010.00911.x</identifier><identifier>PMID: 21375684</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>AIDS ; Alleles ; Blood Banks ; cord blood ; Cord Blood Stem Cell Transplantation - utilization ; Ethnic Groups - genetics ; Fetal Blood - immunology ; Gene Frequency ; Genotype ; HIV Infections - genetics ; HIV Infections - immunology ; HIV Infections - therapy ; Humans ; Infant, Newborn ; Polymerase Chain Reaction ; Receptors, CCR5 - genetics ; Receptors, CCR5 - immunology ; Sequence Analysis, DNA ; stem cell therapy ; transplantation ; virus co‐receptor</subject><ispartof>HIV medicine, 2011-09, Vol.12 (8), p.481-486</ispartof><rights>2011 British HIV Association</rights><rights>2011 British HIV Association.</rights><rights>2011 British HIV Association 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3381-cdb15332d265bbe68171e9ca6778883ff4be1768ff744f08627bc15d94a3a8873</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21375684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gonzalez, G</creatorcontrib><creatorcontrib>Park, SS</creatorcontrib><creatorcontrib>Chen, DW</creatorcontrib><creatorcontrib>Armitage, S</creatorcontrib><creatorcontrib>Shpall, EJ</creatorcontrib><creatorcontrib>Behringer, RR</creatorcontrib><title>Identification and frequency of CCR5Δ32/Δ32 HIV‐resistant cord blood units from Houston area hospitals</title><title>HIV medicine</title><addtitle>HIV Med</addtitle><description>Objectives
The use of umbilical cord blood (CB) that is genetically resistant to HIV infection has been proposed as a novel stem cell therapy for the treatment of patients with AIDS. These genetically unique CB units (CBUs) should be present in public CB banks at a predicted frequency.
Methods
The chemokine (C‐C motif) receptor 5 (CCR5) genotypes of CBUs donated to the M. D. Anderson CB Bank by four Houston area hospitals were determined by polymerase chain reaction (PCR) and DNA sequencing.
Results
The frequency of CCR5Δ32/Δ32 CBUs was consistent with the frequency of the CCR5Δ32 allele in human populations, and was apparently dependent on the ethnic population of the parents of the newborns from whom the CBUs were collected.
Conclusions
Routine genotyping to identify HIV‐resistant CBUs could create a bank of CB‐derived stem/progenitor cells with which to treat HIV infection.</description><subject>AIDS</subject><subject>Alleles</subject><subject>Blood Banks</subject><subject>cord blood</subject><subject>Cord Blood Stem Cell Transplantation - utilization</subject><subject>Ethnic Groups - genetics</subject><subject>Fetal Blood - immunology</subject><subject>Gene Frequency</subject><subject>Genotype</subject><subject>HIV Infections - genetics</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - therapy</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Polymerase Chain Reaction</subject><subject>Receptors, CCR5 - genetics</subject><subject>Receptors, CCR5 - immunology</subject><subject>Sequence Analysis, DNA</subject><subject>stem cell therapy</subject><subject>transplantation</subject><subject>virus co‐receptor</subject><issn>1464-2662</issn><issn>1468-1293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNpVUctqGzEUFSEhTtP8QtGuq7F1pRlJAyUQTBsbDIGQdCs0GimWGY-c0TiNd_2ELPpd_Yh-STR5mEYL3YvO1TnccxDCQMaQzmQ1hpzLDGjJxpSkV0JKgPHjATrZA4cvfZ5RzukIfYpxRQgIVpJjNKLARMFlfoJW89q2vXfe6N6HFuu2xq6z91vbmh0ODk-n18XfP4xOhgvP5j___X7qbPSx122PTehqXDUh1Hjb-j6mv2GNZ2Eb-4GssxovQ9z4XjfxMzpyqdizt3qKbn98v5nOssXV5Xx6scgMYxIyU1dQMEZryouqslyCAFsazYWQUjLn8sqC4NI5keeOSE5FZaCoy1wzLaVgp-j8lXezrda2Nmm_Tjdq0_m17nYqaK8-Iq1fqrvwoHJCQRYyEXx9I-hCMiL2au2jsU2jW5s2U7JkpRAlDFJf_pfaa7z7mwa-vQ788o3d7XEgashRrdQQlxriUkOO6iVH9aiSz6lhz8NMk7o</recordid><startdate>201109</startdate><enddate>201109</enddate><creator>Gonzalez, G</creator><creator>Park, SS</creator><creator>Chen, DW</creator><creator>Armitage, S</creator><creator>Shpall, EJ</creator><creator>Behringer, RR</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201109</creationdate><title>Identification and frequency of CCR5Δ32/Δ32 HIV‐resistant cord blood units from Houston area hospitals</title><author>Gonzalez, G ; Park, SS ; Chen, DW ; Armitage, S ; Shpall, EJ ; Behringer, RR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3381-cdb15332d265bbe68171e9ca6778883ff4be1768ff744f08627bc15d94a3a8873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>AIDS</topic><topic>Alleles</topic><topic>Blood Banks</topic><topic>cord blood</topic><topic>Cord Blood Stem Cell Transplantation - utilization</topic><topic>Ethnic Groups - genetics</topic><topic>Fetal Blood - immunology</topic><topic>Gene Frequency</topic><topic>Genotype</topic><topic>HIV Infections - genetics</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - therapy</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Polymerase Chain Reaction</topic><topic>Receptors, CCR5 - genetics</topic><topic>Receptors, CCR5 - immunology</topic><topic>Sequence Analysis, DNA</topic><topic>stem cell therapy</topic><topic>transplantation</topic><topic>virus co‐receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonzalez, G</creatorcontrib><creatorcontrib>Park, SS</creatorcontrib><creatorcontrib>Chen, DW</creatorcontrib><creatorcontrib>Armitage, S</creatorcontrib><creatorcontrib>Shpall, EJ</creatorcontrib><creatorcontrib>Behringer, RR</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>HIV medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonzalez, G</au><au>Park, SS</au><au>Chen, DW</au><au>Armitage, S</au><au>Shpall, EJ</au><au>Behringer, RR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and frequency of CCR5Δ32/Δ32 HIV‐resistant cord blood units from Houston area hospitals</atitle><jtitle>HIV medicine</jtitle><addtitle>HIV Med</addtitle><date>2011-09</date><risdate>2011</risdate><volume>12</volume><issue>8</issue><spage>481</spage><epage>486</epage><pages>481-486</pages><issn>1464-2662</issn><eissn>1468-1293</eissn><abstract>Objectives
The use of umbilical cord blood (CB) that is genetically resistant to HIV infection has been proposed as a novel stem cell therapy for the treatment of patients with AIDS. These genetically unique CB units (CBUs) should be present in public CB banks at a predicted frequency.
Methods
The chemokine (C‐C motif) receptor 5 (CCR5) genotypes of CBUs donated to the M. D. Anderson CB Bank by four Houston area hospitals were determined by polymerase chain reaction (PCR) and DNA sequencing.
Results
The frequency of CCR5Δ32/Δ32 CBUs was consistent with the frequency of the CCR5Δ32 allele in human populations, and was apparently dependent on the ethnic population of the parents of the newborns from whom the CBUs were collected.
Conclusions
Routine genotyping to identify HIV‐resistant CBUs could create a bank of CB‐derived stem/progenitor cells with which to treat HIV infection.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21375684</pmid><doi>10.1111/j.1468-1293.2010.00911.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1464-2662 |
| ispartof | HIV medicine, 2011-09, Vol.12 (8), p.481-486 |
| issn | 1464-2662 1468-1293 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4021858 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | AIDS Alleles Blood Banks cord blood Cord Blood Stem Cell Transplantation - utilization Ethnic Groups - genetics Fetal Blood - immunology Gene Frequency Genotype HIV Infections - genetics HIV Infections - immunology HIV Infections - therapy Humans Infant, Newborn Polymerase Chain Reaction Receptors, CCR5 - genetics Receptors, CCR5 - immunology Sequence Analysis, DNA stem cell therapy transplantation virus co‐receptor |
| title | Identification and frequency of CCR5Δ32/Δ32 HIV‐resistant cord blood units from Houston area hospitals |
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