Epinephrine stimulation of anion secretion in the Calu-3 serous cell model

Calu-3 is a well-differentiated human bronchial cell line with the characteristics of the serous cells of airway submucosal glands. The submucosal glands play a major role in mucociliary clearance because they secrete electrolytes that facilitate airway hydration. Given the significance of both long...

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Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2014-05, Vol.306 (10), p.L937-L946
Main Authors: Banga, Amiraj, Flaig, Stephanie, Lewis, Shanta, Winfree, Seth, Blazer-Yost, Bonnie L
Format: Article
Language:English
Subjects:
Adrenergic beta-2 Receptor Agonists - pharmacology
Amiloride - pharmacology
Anoctamin-1
Biological Transport
Body fluids
Calcium Ionophores - pharmacology
Cell Line, Tumor
Cells
Cellular biology
Chloride Channels - metabolism
Chlorides - metabolism
Cyclic AMP - metabolism
Cystic Fibrosis Transmembrane Conductance Regulator - metabolism
Electrolytes
Epinephrine - pharmacology
Epinephrine - physiology
Epithelial Sodium Channel Blockers - pharmacology
Hormones
Humans
Ionomycin - pharmacology
Neoplasm Proteins - metabolism
Neurotransmitters
Respiratory diseases
Sodium - metabolism
Tannins - pharmacology
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Summary:Calu-3 is a well-differentiated human bronchial cell line with the characteristics of the serous cells of airway submucosal glands. The submucosal glands play a major role in mucociliary clearance because they secrete electrolytes that facilitate airway hydration. Given the significance of both long- and short-term β-adrenergic receptor agonists in the treatment of respiratory diseases, it is important to determine the role of these receptors and their ligands in normal physiological function. The present studies were designed to characterize the effect of epinephrine, the naturally occurring β-adrenergic receptor agonist, on electrolyte transport of the airway serous cells. Interestingly, epinephrine stimulated two anion secretory channels, the cystic fibrosis transmembrane conductance regulator and a Ca(2+)-activated Cl(-) channel, with the characteristics of transmembrane protein 16A, thereby potentially altering mucociliary clearance via multiple channels. Consistent with the dual channel activation, epinephrine treatment resulted in increases in both intracellular cAMP and Ca(2+). Furthermore, the present results extend previous reports indicating that the two anion channels are functionally linked.
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00190.2013