Estimating telomere length from whole genome sequence data

Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot...

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Bibliographic Details
Published in:Nucleic acids research 2014-05, Vol.42 (9), p.e75-e75
Main Authors: Ding, Zhihao, Mangino, Massimo, Aviv, Abraham, Spector, Tim, Durbin, Richard
Format: Article
Language:English
Subjects:
Adult
Aged
Base Sequence
Cross-Sectional Studies
Exome
Genome, Human
Humans
Leukocytes - physiology
Methods Online
Middle Aged
Models, Genetic
Repetitive Sequences, Nucleic Acid
Sequence Analysis, DNA
Software
Telomere - genetics
Telomere Homeostasis
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Summary:Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gku181